CAS6_PECAS
ID CAS6_PECAS Reviewed; 184 AA.
AC Q6D0W5;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=CRISPR-associated endonuclease Cas6f/Csy4;
DE EC=3.1.-.-;
DE AltName: Full=crRNA endonuclease;
GN Name=cas6f; Synonyms=csy4; OrderedLocusNames=ECA3684;
OS Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS carotovora subsp. atroseptica).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Pectobacterium.
OX NCBI_TaxID=218491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT subsp. atroseptica and characterization of virulence factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
RN [2]
RP FUNCTION IN CRRNA FORMATION, SUBUNIT, INDUCTION, OPERON STRUCTURE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=21519197; DOI=10.4161/rna.8.3.15190;
RA Przybilski R., Richter C., Gristwood T., Clulow J.S., Vercoe R.B.,
RA Fineran P.C.;
RT "Csy4 is responsible for CRISPR RNA processing in Pectobacterium
RT atrosepticum.";
RL RNA Biol. 8:517-528(2011).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=23226499; DOI=10.1371/journal.pone.0049549;
RA Richter C., Gristwood T., Clulow J.S., Fineran P.C.;
RT "In vivo protein interactions and complex formation in the Pectobacterium
RT atrosepticum subtype I-F CRISPR/Cas System.";
RL PLoS ONE 7:E49549-E49549(2012).
RN [4]
RP FUNCTION IN CRRNA FORMATION, BIOTECHNOLOGY, AND DISRUPTION PHENOTYPE.
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=23637624; DOI=10.1371/journal.pgen.1003454;
RA Vercoe R.B., Chang J.T., Dy R.L., Taylor C., Gristwood T., Clulow J.S.,
RA Richter C., Przybilski R., Pitman A.R., Fineran P.C.;
RT "Cytotoxic chromosomal targeting by CRISPR/Cas systems can reshape
RT bacterial genomes and expel or remodel pathogenicity islands.";
RL PLoS Genet. 9:E1003454-E1003454(2013).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC Processes pre-crRNA into individual crRNA units.
CC {ECO:0000269|PubMed:21519197, ECO:0000269|PubMed:23637624}.
CC -!- SUBUNIT: Homodimer. Part of the probable Csy ribonucleoprotein complex
CC with cys1, cys2, csy3, cas6f and crRNA. {ECO:0000269|PubMed:21519197,
CC ECO:0000269|PubMed:23226499}.
CC -!- INDUCTION: Expressed in late exponential phase (other phases not
CC tested); part of a large cas-CRISPR3 polycistronic operon.
CC {ECO:0000269|PubMed:21519197}.
CC -!- DISRUPTION PHENOTYPE: Loss of crRNA processing (PubMed:21519197), no
CC chromosomal targeting (PubMed:23637624). {ECO:0000269|PubMed:21519197,
CC ECO:0000269|PubMed:23637624}.
CC -!- BIOTECHNOLOGY: If the spacer DNA has a perfect match in the chromosome
CC then toxicity results. Suppression of the toxic effects occurs via
CC mutations in the CRISPR/Cas machinery, or via target deletion, which
CC might contribute to genome plasticity. This CRISPR/Cas system can be
CC used to remove genomic islands, and possibly other genomic regions.
CC {ECO:0000269|PubMed:23637624}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas6
CC family. Cas6f/Csy4, subtype I-F/Ypest subfamily. {ECO:0000305}.
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DR EMBL; BX950851; CAG76582.1; -; Genomic_DNA.
DR RefSeq; WP_011095184.1; NC_004547.2.
DR AlphaFoldDB; Q6D0W5; -.
DR SMR; Q6D0W5; -.
DR STRING; 218491.ECA3684; -.
DR EnsemblBacteria; CAG76582; CAG76582; ECA3684.
DR KEGG; eca:ECA3684; -.
DR PATRIC; fig|218491.5.peg.3736; -.
DR eggNOG; ENOG5032RVU; Bacteria.
DR HOGENOM; CLU_108958_0_0_6; -.
DR OMA; HYIEIRV; -.
DR OrthoDB; 1776101at2; -.
DR Proteomes; UP000007966; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:InterPro.
DR CDD; cd09739; Cas6_I-F; 1.
DR Gene3D; 3.30.70.2540; -; 1.
DR InterPro; IPR013396; CRISPR-assoc_prot_Csy4.
DR InterPro; IPR042564; CRISPR-Cas6/Csy4_sf.
DR Pfam; PF09618; Cas_Csy4; 1.
DR TIGRFAMs; TIGR02563; cas_Csy4; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW RNA-binding.
FT CHAIN 1..184
FT /note="CRISPR-associated endonuclease Cas6f/Csy4"
FT /id="PRO_0000430242"
SQ SEQUENCE 184 AA; 20459 MW; 347B735A28FD5EA4 CRC64;
MDHYIDIRVQ PDPEFTASQL LNALFAKLHR VLGQLANGKI GISFPEVGKT LGECLRLHGT
EDALSTLEKT SWLKGLRDYT QVSECKVVPN GVKFRTVRRV QLKSSAERLR RRSVSKGWLT
AAEAAARIPD AVEKRSALPF VQIKSLSNGQ MFFVFVEHGP LQNAPTAGRF SSYGLSTEAT
VPWF
