ID   Y525_METKA              Reviewed;         196 AA.
AC   P50100;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2002, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Uncharacterized protein MK0525;
DE   AltName: Full=OrfX;
GN   OrderedLocusNames=MK0525;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172.
RX   PubMed=8522533; DOI=10.1128/jb.177.24.7238-7244.1995;
RA   Noelling J., Pihl T.D., Reeve J.N.;
RT   "Cloning, sequencing, and growth phase-dependent transcription of the
RT   coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-
RT   encoding genes from Methanobacterium thermoautotrophicum delta H and
RT   Methanopyrus kandleri.";
RL   J. Bacteriol. 177:7238-7244(1995).
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DR   EMBL; AE009439; AAM01740.1; -; Genomic_DNA.
DR   EMBL; U31567; AAA92086.1; -; Genomic_DNA.
DR   AlphaFoldDB; P50100; -.
DR   SMR; P50100; -.
DR   STRING; 190192.MK0525; -.
DR   EnsemblBacteria; AAM01740; AAM01740; MK0525.
DR   KEGG; mka:MK0525; -.
DR   PATRIC; fig|190192.8.peg.559; -.
DR   HOGENOM; CLU_040681_7_0_2; -.
DR   OMA; YGICELC; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.10.580.10; -; 1.
DR   InterPro; IPR044065; ACP_MB.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   Pfam; PF00571; CBS; 2.
DR   SMART; SM00116; CBS; 2.
DR   SUPFAM; SSF54631; SSF54631; 1.
DR   PROSITE; PS51901; ACP_MB; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   4: Predicted;
KW   CBS domain; Iron; Metal-binding; Reference proteome; Repeat; Zinc.
FT   CHAIN           1..196
FT                   /note="Uncharacterized protein MK0525"
FT                   /id="PRO_0000106973"
FT   DOMAIN          10..69
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          76..132
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          153..187
FT                   /note="ACP-type MB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         158
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         158
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         161
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         161
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         177
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         177
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         180
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   BINDING         180
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01249"
FT   CONFLICT        3
FT                   /note="S -> T (in Ref. 2; AAA92086)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   196 AA;  22197 MW;  9AC4960413652890 CRC64;
     MRSVSVGEVA RRDVITGSPT ETAVEIAYKM REHGIGSVVI VNEKDEPIGI ITERDLVIKV
     VSQGKNPDEV IARDIMSQPV ITVEEDMEVN EAVKLMVDKG IRRLPIVDDN GKLIGIVTMQ
     DILQVEPYLV ATIEEEMKKF QEELEELEEV SEIIEGVCDL CETYSEELRF VDGVWVCPEC
     YEDILGREIE DRELEE