ID   Y5261_AGRFC             Reviewed;         553 AA.
AC   Q8UK62; Q7D3H8;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Uncharacterized esterase Atu5261 {ECO:0000305};
DE            EC=3.1.1.- {ECO:0000305};
DE   Flags: Precursor;
GN   OrderedLocusNames=Atu5261; ORFNames=AGR_pAT_374;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid AT.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- SIMILARITY: Belongs to the tannase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK90635.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE007872; AAK90635.2; ALT_INIT; Genomic_DNA.
DR   PIR; AH3191; AH3191.
DR   RefSeq; NP_396194.2; NC_003064.2.
DR   AlphaFoldDB; Q8UK62; -.
DR   SMR; Q8UK62; -.
DR   STRING; 176299.Atu5261; -.
DR   ESTHER; agrt5-y5261; Tannase.
DR   EnsemblBacteria; AAK90635; AAK90635; Atu5261.
DR   KEGG; atu:Atu5261; -.
DR   PATRIC; fig|176299.10.peg.4936; -.
DR   eggNOG; COG0627; Bacteria.
DR   HOGENOM; CLU_014819_4_0_5; -.
DR   OMA; LTPMAKN; -.
DR   BioCyc; AGRO:ATU5261-MON; -.
DR   Proteomes; UP000000813; Plasmid At.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR011118; Tannase/feruloyl_esterase.
DR   PANTHER; PTHR33938; PTHR33938; 1.
DR   Pfam; PF07519; Tannase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Calcium; Disulfide bond; Hydrolase; Metal-binding; Plasmid;
KW   Reference proteome; Serine esterase; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..553
FT                   /note="Uncharacterized esterase Atu5261"
FT                   /id="PRO_0000221473"
FT   ACT_SITE        207
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   ACT_SITE        444
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   ACT_SITE        482
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   BINDING         276
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   BINDING         279
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   BINDING         281
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   BINDING         283
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   BINDING         285
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   DISULFID        275..292
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
FT   DISULFID        528..550
FT                   /evidence="ECO:0000250|UniProtKB:Q2UP89"
SQ   SEQUENCE   553 AA;  59523 MW;  85F099224983A10D CRC64;
     MEDIMTSLLV ATSRVVVTIS LAYVPVKSAF AAPQNAAGLC SAIAASSIPA SRISLPTTGA
     KIQSATLIGG SDPANQNGEY CKVIGQIKPV DPKAPDIIWQ VNLPSVWNGK MLQYGGGGYN
     GSIPPTTDKT TLGLDVVPTP LTQGYVTFGS DSGHQAPNAD DASFAKNDEA MLNYGYMHIK
     KVLDVAKVLV TERYEKPVTR VYFQGGSTGG REGLTAASRW PESYDGILTN YPTANFVGLR
     LWGAGLARAV YDDKSAGWIP PKLVERISKE ALKSCDGLDG VEDGLVGNMQ QCRAQSAVLV
     QSLACKSDVT GNPDDCLTQA QIERTLKIYH EGYSLPYQLA NGINTYPGYN SLEGIMMQLG
     SEPQMRTPPV SGPNAHHSSR SFEFLQNFVQ RDQPLNLLSF DIREPGKLKD RIVELSDVIG
     ATRTDWSTFS DRGGKIIWLQ GNDDPSVSPL GNAKLFESIV AKMGADKVKG FMRFFLVPGL
     AHGGGRFSPT WDNLAALDNW VEHDVPPSNP VVVDATKSST KGRSRPLCEY PSWPKYKGDG
     DVAIASSFSC ADD