CAS6_PYRHO
ID CAS6_PYRHO Reviewed; 267 AA.
AC O58931;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=CRISPR-associated endoribonuclease Cas6;
DE EC=3.1.-.-;
DE AltName: Full=PhCas6;
GN Name=cas6; OrderedLocusNames=PH1252;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
RN [2]
RP FUNCTION AS AN ENDORIBONUCLEASE.
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=22238224; DOI=10.1002/pro.2028;
RA Wang R., Zheng H., Preamplume G., Shao Y., Li H.;
RT "The impact of CRISPR repeat sequence on structures of a Cas6 protein-RNA
RT complex.";
RL Protein Sci. 21:405-417(2012).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC protein processes pre-crRNA into individual crRNA units.
CC {ECO:0000269|PubMed:22238224}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000305}.
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DR EMBL; BA000001; BAA30353.1; -; Genomic_DNA.
DR PIR; G71069; G71069.
DR AlphaFoldDB; O58931; -.
DR SMR; O58931; -.
DR STRING; 70601.3257670; -.
DR EnsemblBacteria; BAA30353; BAA30353; BAA30353.
DR KEGG; pho:PH1252; -.
DR eggNOG; arCOG04342; Archaea.
DR OMA; FGMVKVD; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1890; -; 1.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR PANTHER; PTHR36984; PTHR36984; 1.
DR Pfam; PF01881; Cas_Cas6; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
DR TIGRFAMs; TIGR01877; cas_cas6; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Endonuclease; Hydrolase; Nuclease; RNA-binding.
FT CHAIN 1..267
FT /note="CRISPR-associated endoribonuclease Cas6"
FT /id="PRO_0000417972"
FT ACT_SITE 32
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT ACT_SITE 47
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
SQ SEQUENCE 267 AA; 31523 MW; 7D15E2BFD905F4A5 CRC64;
MIIMRFLIKV KPEERKFRVP YNHQYFLQGL IYNRIKLTNP RLSTFLHETK GPKLFTYSLF
MTERREHPKG LPYFLGYRRG FFYFSTCIPE IAEAFITGLF REPEITLWGE KFYLEEVKTL
KEPKKFSGST FITLSPIAVT MEKGGKRYDV SPLEEEFYAL IRENLKDKYV MIKGEKPPDD
FEMEIIAAKP KRFEVKPGIY QMAWHLVFRA YGDDELIRVG YVVGFGEKNS LGFGMVKVDE
QRKRRKNIGS FQESMSFNEN RELETGT
