CAS7_HALVD
ID CAS7_HALVD Reviewed; 340 AA.
AC D4GQN6;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 25-MAY-2022, entry version 51.
DE RecName: Full=CRISPR-associated protein Cas7 {ECO:0000303|PubMed:24459147};
GN Name=cas7 {ECO:0000303|PubMed:24459147}; Synonyms=csh2;
GN OrderedLocusNames=HVO_A0207; ORFNames=C498_09711;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG Plasmid pHV4.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=DS2 / DS70 / H26;
RX PubMed=22767603; DOI=10.1074/jbc.m112.377002;
RA Fischer S., Maier L.K., Stoll B., Brendel J., Fischer E., Pfeiffer F.,
RA Dyall-Smith M., Marchfelder A.;
RT "An archaeal immune system can detect multiple protospacer adjacent motifs
RT (PAMs) to target invader DNA.";
RL J. Biol. Chem. 287:33351-33363(2012).
RN [4]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=DS2 / DS70 / H119;
RX PubMed=24459147; DOI=10.1074/jbc.m113.508184;
RA Brendel J., Stoll B., Lange S.J., Sharma K., Lenz C., Stachler A.E.,
RA Maier L.K., Richter H., Nickel L., Schmitz R.A., Randau L., Allers T.,
RA Urlaub H., Backofen R., Marchfelder A.;
RT "A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and
RT stability of clustered regularly interspaced short palindromic repeats
RT (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.";
RL J. Biol. Chem. 289:7164-7177(2014).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid
CC targeted by CRISPR locus P1 transform wild-type cells very poorly
CC (PubMed:22767603). This protein helps process or stabilize pre-crRNA
CC into individual crRNA units, in vivo Cas6 and Cas7 are also required
CC for optimal crRNA processing and/or stability (PubMed:24459147).
CC {ECO:0000269|PubMed:22767603, ECO:0000269|PubMed:24459147}.
CC -!- SUBUNIT: Component of the Cascade-like complex (Cascade I-B), composed
CC of Cas5, Cas6, Cas7 and crRNA. {ECO:0000269|PubMed:24459147}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24459147}.
CC -!- DISRUPTION PHENOTYPE: Loss of the 8 Cas genes in this locus (cas1,
CC cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR
CC interference against plasmid targeted by this CRISPR locus, i.e.
CC plasmid is not destroyed by CRISPR. {ECO:0000269|PubMed:22767603}.
CC -!- MISCELLANEOUS: There are 3 CRISPR RNA loci in this organism and a
CC single cas gene locus. A CRISPR-Cas type I-B system.
CC {ECO:0000303|PubMed:20333302, ECO:0000303|PubMed:24459147}.
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DR EMBL; CP001955; ADE01855.1; -; Genomic_DNA.
DR EMBL; AOHU01000052; ELY32093.1; -; Genomic_DNA.
DR RefSeq; WP_004043127.1; NZ_AOHU01000052.1.
DR AlphaFoldDB; D4GQN6; -.
DR SMR; D4GQN6; -.
DR STRING; 309800.C498_09711; -.
DR EnsemblBacteria; ADE01855; ADE01855; HVO_A0207.
DR EnsemblBacteria; ELY32093; ELY32093; C498_09711.
DR GeneID; 8923320; -.
DR KEGG; hvo:HVO_A0207; -.
DR PATRIC; fig|309800.29.peg.1897; -.
DR eggNOG; arCOG02757; Archaea.
DR HOGENOM; CLU_071770_0_0_2; -.
DR OMA; YDFVLLF; -.
DR OrthoDB; 47282at2157; -.
DR Proteomes; UP000008243; Plasmid pHV4.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:InterPro.
DR InterPro; IPR006482; Cas7_Csh2/Csh2.
DR InterPro; IPR013419; CRISPR-assoc_prot_Csh2.
DR Pfam; PF05107; Cas_Cas7; 1.
DR TIGRFAMs; TIGR02590; cas_Csh2; 1.
DR TIGRFAMs; TIGR01595; cas_CT1132; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Cytoplasm; Plasmid; Reference proteome.
FT CHAIN 1..340
FT /note="CRISPR-associated protein Cas7"
FT /id="PRO_0000432157"
SQ SEQUENCE 340 AA; 38184 MW; 09E7E81D4311A827 CRC64;
MTTLNRSELL FVYDAQDCNP NGNPIGDNRP RRDPDTGQGI ITDVRLKRYL RDQLQDDGFD
IYVKKTEGRS HTRTKLIKDV LGGVDDADDL EDLGDVAEAF LDAATDVRYF GSTLSFEASD
KDEDEAFRDA LNSSLPNNYQ GPVQFLPSKS LNEVEENEEY DSLTSVISTG EGNRQGGFDL
DDKRIKYGIF PFWGLVDNNG AESTNLSQAD VERLDTLCWR ALKNQTTSRS KLGQEPRLYV
RVEYEEGNYH VGGLQNLLEL GDDSSESLRS VKDVVVDVTE LVETLERVAD RIDTLHVVGD
GRLELDIGDE TIRADEFWSH LSEAGHDVHE IDVLNERDLA
