Y5325_ARATH
ID Y5325_ARATH Reviewed; 592 AA.
AC Q9LYW0;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=BTB/POZ domain-containing protein At5g03250;
GN OrderedLocusNames=At5g03250; ORFNames=F15A17_280, MOK16.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00982}.
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DR EMBL; AB005240; BAB08385.1; -; Genomic_DNA.
DR EMBL; AL163002; CAB86092.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90577.1; -; Genomic_DNA.
DR EMBL; AK226794; BAE98892.1; -; mRNA.
DR PIR; T48346; T48346.
DR RefSeq; NP_195945.1; NM_120403.3.
DR AlphaFoldDB; Q9LYW0; -.
DR SMR; Q9LYW0; -.
DR PaxDb; Q9LYW0; -.
DR PRIDE; Q9LYW0; -.
DR ProteomicsDB; 243133; -.
DR EnsemblPlants; AT5G03250.1; AT5G03250.1; AT5G03250.
DR GeneID; 831894; -.
DR Gramene; AT5G03250.1; AT5G03250.1; AT5G03250.
DR KEGG; ath:AT5G03250; -.
DR Araport; AT5G03250; -.
DR TAIR; locus:2143558; AT5G03250.
DR eggNOG; ENOG502QR2D; Eukaryota.
DR HOGENOM; CLU_005994_6_2_1; -.
DR InParanoid; Q9LYW0; -.
DR OMA; RILEHFM; -.
DR OrthoDB; 481589at2759; -.
DR PhylomeDB; Q9LYW0; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9LYW0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LYW0; baseline and differential.
DR Genevisible; Q9LYW0; AT.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043454; NPH3/RPT2-like.
DR InterPro; IPR027356; NPH3_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR32370; PTHR32370; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF03000; NPH3; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS51649; NPH3; 1.
PE 2: Evidence at transcript level;
KW Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..592
FT /note="BTB/POZ domain-containing protein At5g03250"
FT /id="PRO_0000409582"
FT DOMAIN 28..98
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 217..502
FT /note="NPH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT MOD_RES 443
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9FMF5"
SQ SEQUENCE 592 AA; 66343 MW; 7D575596EF8E3F06 CRC64;
MAFMRLGSKS EAFHREGQTW LCTTGLVSDV TIEVGDMKFH LHKFPLLSRS GLLERLIEES
STDDGSGCVL SLDEIPGGGK TFELVTKFCY GVKIELTAFN VVSLRCAAEY LEMTDNYGEG
NLVGMTETFL NEVFGNWTDS IKALQTCEEV IDYAEDLHII SRCVDSLAVK ACADPSLFNW
PVGGGKNATS GQNTEDESHL WNGISASGKM LQHTGEDWWF DDASFLSLPL FKRLITAIEA
RGMKLENIAM AVMYYTRKHV PLMNRQVNMD EQVIETPNPS EEDQKTCLEE IVGLLPSKKG
VNPTKFLLRL LQTAMVLHAS QSSRENLERR IGNQLDQAAL VDLLIPNMGY SETLYDVECV
LRMIEQFVSS TEQAGIVPSP CIIEEGHLVK DGADLLTPTT LVATLVDGYL AEVAPDVNLK
LAKFEAIAAA IPDYARPLDD GVYHAIDVYL KAHPWITDSE REHICRLMNC QKLSLEASTH
AAQNERLPLR VIVQVLFFEQ LRLRTSVSGW FFVSENLDNP DNQHGANGGL LKPRGENVRE
RVSELEKECM NMKQELHKLV RTKRSWKNFT RKLNFKKKSE CCKPKDQATP AI
