ID   CAS8B_HALVD             Reviewed;         717 AA.
AC   D4GQN5;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=CRISPR-associated protein Cas8b {ECO:0000303|PubMed:24459147};
GN   Name=cas8b {ECO:0000303|PubMed:24459147}; OrderedLocusNames=HVO_A0206;
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG   Plasmid pHV4.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2 {ECO:0000312|Proteomes:UP000008243};
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DS2 / DS70 / H26;
RX   PubMed=22767603; DOI=10.1074/jbc.m112.377002;
RA   Fischer S., Maier L.K., Stoll B., Brendel J., Fischer E., Pfeiffer F.,
RA   Dyall-Smith M., Marchfelder A.;
RT   "An archaeal immune system can detect multiple protospacer adjacent motifs
RT   (PAMs) to target invader DNA.";
RL   J. Biol. Chem. 287:33351-33363(2012).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=DS2 / DS70 / H119;
RX   PubMed=24459147; DOI=10.1074/jbc.m113.508184;
RA   Brendel J., Stoll B., Lange S.J., Sharma K., Lenz C., Stachler A.E.,
RA   Maier L.K., Richter H., Nickel L., Schmitz R.A., Randau L., Allers T.,
RA   Urlaub H., Backofen R., Marchfelder A.;
RT   "A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and
RT   stability of clustered regularly interspaced short palindromic repeats
RT   (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.";
RL   J. Biol. Chem. 289:7164-7177(2014).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid
CC       targeted by CRISPR locus P1 transform wild-type cells very poorly
CC       (PubMed:22767603). This subunit might be involved in stabilizing crRNA
CC       (PubMed:24459147). {ECO:0000269|PubMed:22767603,
CC       ECO:0000269|PubMed:24459147}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24459147}.
CC   -!- DISRUPTION PHENOTYPE: Loss of the 8 Cas genes in this locus (cas1,
CC       cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR
CC       interference against plasmid targeted by this CRISPR locus, i.e.
CC       plasmid is not destroyed by CRISPR. Disruption of this single gene also
CC       leads to loss of CRISPR interference. {ECO:0000269|PubMed:22767603}.
CC   -!- MISCELLANEOUS: There are 3 CRISPR RNA loci in this organism and a
CC       single cas gene locus. A CRISPR-Cas type I-B system.
CC       {ECO:0000303|PubMed:20333302, ECO:0000303|PubMed:24459147}.
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DR   EMBL; CP001955; ADE01723.1; -; Genomic_DNA.
DR   AlphaFoldDB; D4GQN5; -.
DR   STRING; 309800.C498_09706; -.
DR   PRIDE; D4GQN5; -.
DR   EnsemblBacteria; ADE01723; ADE01723; HVO_A0206.
DR   KEGG; hvo:HVO_A0206; -.
DR   eggNOG; arCOG05124; Archaea.
DR   HOGENOM; CLU_391622_0_0_2; -.
DR   OMA; IDHSVTH; -.
DR   Proteomes; UP000008243; Plasmid pHV4.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013389; CRISPR-assoc_prot_Cas8b.
DR   InterPro; IPR013420; CRISPR-assoc_prot_Csh1_C.
DR   Pfam; PF09484; Cas_TM1802; 1.
DR   TIGRFAMs; TIGR02591; cas_Csh1; 1.
PE   4: Predicted;
KW   Antiviral defense; Cytoplasm; Plasmid; Reference proteome.
FT   CHAIN           1..717
FT                   /note="CRISPR-associated protein Cas8b"
FT                   /id="PRO_0000432158"
FT   REGION          263..283
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          698..717
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   717 AA;  79352 MW;  CCD4056CA0C4CD49 CRC64;
     MTGPDIDDFE NALNAFWHGR PPASLEDVMA LYGVLAVAES GGELYGTDSK LEPFVDDGRL
     VTIDIDLTGE TPNVSDPKVD TLRVEDVSKL RYAHKSSGRG AKYSLTQIGS KNGNDAEGVA
     STILGRVRSW TTQDSVRSVT GEDGHPDGWI VEELAAVFEK GSDTLEALEE AIKALLPPDE
     SLPTVITVRL RLDAGRLSHG EESGPRWFWP AELDVLEEAM KRYATANAAD KNVESGDGIS
     EGESVGLVTD RVERVVGTPD NPIGVFSVKH PDAQPGLRQD QSWRNYPVGA DTAMLFSKGQ
     DLVETCVLRR GGVETYALPY FAGELTPLKA QSLYGAIQSL DRESDYDDSG GSPLARVTYE
     LRESDDETLQ ELAKTELRFY TITLPIGDDK NVIAEEPAAP VYWVSELADA LAQTVHGPTL
     NPERGGFAPY DNWSLLELAT EDFEESRKFG FYRIVGHQFT DSAFAYRGDD EDDDFRRVVD
     HRLIAGVPLD ASMLFDEYLR RYHDESEGGD LPPHQIVAQQ LVHLETLSRA GLLNGLDVPI
     EPPTMTTETE TETDFDTTSL PAIREHRLES FLDRPLFEAP ARRAAALAGV LVGQVSWHQE
     SERNVGRPLD AQTKGDQLTK NSLENALTSA LEKAKVYALD SEYRSDRDML FPETVDRLLE
     TTEDMPSAWP IEKRELQFCY VLGHAHGRRS MPVAFDLHEK EDEDDQDTEE PAESTTN