ID   CAS8_THETK              Reviewed;         361 AA.
AC   G4RJZ5;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=CRISPR system associated protein Cas8;
GN   Name=cas8a2; OrderedLocusNames=TTX_1255;
OS   Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435
OS   / Kra 1).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Thermoproteus.
OX   NCBI_TaxID=768679;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1;
RX   PubMed=22003381; DOI=10.1371/journal.pone.0024222;
RA   Siebers B., Zaparty M., Raddatz G., Tjaden B., Albers S.V., Bell S.D.,
RA   Blombach F., Kletzin A., Kyrpides N., Lanz C., Plagens A., Rampp M.,
RA   Rosinus A., von Jan M., Makarova K.S., Klenk H.P., Schuster S.C.,
RA   Hensel R.;
RT   "The complete genome sequence of Thermoproteus tenax: a physiologically
RT   versatile member of the Crenarchaeota.";
RL   PLoS ONE 6:E24222-E24222(2011).
RN   [2]
RP   SUBUNIT, INDUCTION, AND OPERON STRUCTURE.
RC   STRAIN=ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1;
RX   PubMed=22408157; DOI=10.1128/jb.00206-12;
RA   Plagens A., Tjaden B., Hagemann A., Randau L., Hensel R.;
RT   "Characterization of the CRISPR/Cas subtype I-A system of the
RT   hyperthermophilic crenarchaeon Thermoproteus tenax.";
RL   J. Bacteriol. 194:2491-2500(2012).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer (By similarity). Can form a Cascade complex with Csa5,
CC       Cas7, Cas5a, Cas3 and Cas3'. {ECO:0000250,
CC       ECO:0000269|PubMed:22408157}.
CC   -!- INDUCTION: Repressed by 5 J/m2 ultraviolet light and 50 mM NaCl,
CC       slightly induced by 20 J/m2 ultraviolet light, 100 and 150 mM Nacl.
CC       Member of the csa5-cas7-cas5a-cas3-cas3'-cas8a2 operon.
CC       {ECO:0000269|PubMed:22408157}.
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DR   EMBL; FN869859; CCC81890.1; -; Genomic_DNA.
DR   RefSeq; WP_014127145.1; NC_016070.1.
DR   AlphaFoldDB; G4RJZ5; -.
DR   STRING; 768679.TTX_1255; -.
DR   EnsemblBacteria; CCC81890; CCC81890; TTX_1255.
DR   GeneID; 11262135; -.
DR   KEGG; ttn:TTX_1255; -.
DR   PATRIC; fig|768679.9.peg.1268; -.
DR   eggNOG; arCOG01441; Archaea.
DR   HOGENOM; CLU_751430_0_0_2; -.
DR   OMA; MAGLVEM; -.
DR   OrthoDB; 138070at2157; -.
DR   Proteomes; UP000002654; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR010184; CRISPR-assoc_prot_MJ0385.
DR   Pfam; PF09703; Cas_Csa4; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome.
FT   CHAIN           1..361
FT                   /note="CRISPR system associated protein Cas8"
FT                   /id="PRO_0000422230"
SQ   SEQUENCE   361 AA;  40403 MW;  2FBF674A07609320 CRC64;
     MTQCPQEMIT PGHGIIADAL IMHGLIKILH IEGKMEGWAE RRGERFVVCA ERPDLNWIDQ
     WEPMELLEIA AEFKAKGKDR ASEEEEQRPY FGLVHDLSRS TVDPGNFSSW ISDVREALYA
     LKADFDLSEE HKEKRGEGRA RSKKRGYYTL YLPLSGVYGK YVVENYGIRQ SQYAVCATCF
     ALSTLGYIYG TVKARVERRS SSGGGHDVFN LTFIPRERTS LRSLMALQRM AGLVEMRPGD
     LNELGAVVYM LSVGETIYAV REGVDILVWV TQRAGNFQRT VGVNIFRGDR LLEAIAEIKY
     RAPSWPKIAR QLGSSLNVLG EYLAFGGDVY HVIRSVMADL GRKGGKLAGL EGLAEALKKI
     G