Y540_THET8
ID Y540_THET8 Reviewed; 336 AA.
AC Q5SKV3;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Uncharacterized PIN and TRAM-domain containing protein TTHA0540;
DE AltName: Full=Putative RNase TTHA0540;
DE EC=3.1.-.-;
DE Flags: Precursor;
GN OrderedLocusNames=TTHA0540;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 138-269.
RA Chang C., Li H., Clancy S., Joachimiak A.;
RT "Crystal structure of a domain of functionally unknown protein from Thermus
RT thermophilus HB8.";
RL Submitted (SEP-2009) to the PDB data bank.
CC -!- FUNCTION: Part of a toxin-antitoxin (TA) system. An RNase.
CC {ECO:0000305}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
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DR EMBL; AP008226; BAD70363.1; -; Genomic_DNA.
DR RefSeq; WP_011228012.1; NC_006461.1.
DR RefSeq; YP_143806.1; NC_006461.1.
DR PDB; 3IX7; X-ray; 2.15 A; A/B=138-269.
DR PDBsum; 3IX7; -.
DR AlphaFoldDB; Q5SKV3; -.
DR SMR; Q5SKV3; -.
DR STRING; 300852.55771922; -.
DR DNASU; 3168801; -.
DR EnsemblBacteria; BAD70363; BAD70363; BAD70363.
DR GeneID; 3168801; -.
DR KEGG; ttj:TTHA0540; -.
DR PATRIC; fig|300852.9.peg.539; -.
DR eggNOG; COG4956; Bacteria.
DR HOGENOM; CLU_050839_0_0_0; -.
DR OMA; EGPLWVP; -.
DR PhylomeDB; Q5SKV3; -.
DR EvolutionaryTrace; Q5SKV3; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002792; TRAM_dom.
DR Pfam; PF01938; TRAM; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR PROSITE; PS50926; TRAM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Membrane; Metal-binding; Nuclease;
KW Reference proteome; Signal; Toxin-antitoxin system; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..336
FT /note="Uncharacterized PIN and TRAM-domain containing
FT protein TTHA0540"
FT /id="PRO_0000407901"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 144..255
FT /note="PINc"
FT DOMAIN 267..328
FT /note="TRAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT BINDING 222
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255"
FT STRAND 143..145
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 147..152
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 154..159
FT /evidence="ECO:0007829|PDB:3IX7"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:3IX7"
FT STRAND 168..171
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 172..182
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 187..206
FT /evidence="ECO:0007829|PDB:3IX7"
FT STRAND 209..212
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 221..231
FT /evidence="ECO:0007829|PDB:3IX7"
FT STRAND 235..239
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 241..249
FT /evidence="ECO:0007829|PDB:3IX7"
FT STRAND 254..256
FT /evidence="ECO:0007829|PDB:3IX7"
FT HELIX 257..263
FT /evidence="ECO:0007829|PDB:3IX7"
SQ SEQUENCE 336 AA; 36378 MW; 3BCC21DEA4DE510A CRC64;
MKTRHLVYLA FALLGLGLAG LLEDWGLLPQ SPSLLSLNRL YLALAGLLTG LLLGPRLEGA
LEARLKRLRS LPPEVVVATT LGSTIGLLLA VLLTTLLAQV PGFSPVHSLL LALGLVALFV
YLALGYRAYF RLPEPKPAPR GGKVLDTSVL VDGRVAEVAA VGFLEGPLWV PHFVLKELQH
FADSQDPLRR AKGRRGLETL ERLREAAPLE VLETTPKGES VDEKLLFLAR DLEAALVTND
HALLQMARIY GVKALSIQAL AQALRPQLQV GDTLKLLILK EGKEPHQGVG YLEDGSMVVV
DGGSRYRGQE IEVVVTQAIQ TQVGRLFFAR PAQGAQ
