Y5410_ARATH
ID Y5410_ARATH Reviewed; 614 AA.
AC Q9FL63;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Inactive leucine-rich repeat receptor-like serine/threonine-protein kinase At5g24100;
DE Flags: Precursor;
GN OrderedLocusNames=At5g24100; ORFNames=MZF18.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=20064227; DOI=10.1186/1471-2164-11-19;
RA Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.;
RT "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-
RT like protein kinase genes in Arabidopsis thaliana.";
RL BMC Genomics 11:19-19(2010).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- INTERACTION:
CC Q9FL63; C0LGJ1: At1g74360; NbExp=3; IntAct=EBI-20657062, EBI-20652666;
CC Q9FL63; C0LGS3: At4g37250; NbExp=3; IntAct=EBI-20657062, EBI-16955335;
CC Q9FL63; C0LGQ4: MDIS2; NbExp=2; IntAct=EBI-20657062, EBI-20665429;
CC Q9FL63; Q93ZS4: NIK3; NbExp=3; IntAct=EBI-20657062, EBI-17121474;
CC Q9FL63; Q9ZVR7: PSKR1; NbExp=3; IntAct=EBI-20657062, EBI-16172949;
CC Q9FL63; Q9FN37: PSKR2; NbExp=2; IntAct=EBI-20657062, EBI-16902047;
CC Q9FL63; Q9M2R4: T10K17.40; NbExp=3; IntAct=EBI-20657062, EBI-20657109;
CC Q9FL63; P43298: TMK1; NbExp=3; IntAct=EBI-20657062, EBI-2023970;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; AB010696; BAB11570.1; -; Genomic_DNA.
DR EMBL; AB009056; BAB11570.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED93256.1; -; Genomic_DNA.
DR EMBL; BT005989; AAO64924.1; -; mRNA.
DR EMBL; FJ708782; ACN59373.1; -; mRNA.
DR EMBL; AK227407; BAE99411.1; -; mRNA.
DR RefSeq; NP_197798.1; NM_122315.4.
DR AlphaFoldDB; Q9FL63; -.
DR SMR; Q9FL63; -.
DR BioGRID; 17750; 51.
DR IntAct; Q9FL63; 53.
DR STRING; 3702.AT5G24100.1; -.
DR PaxDb; Q9FL63; -.
DR PRIDE; Q9FL63; -.
DR ProteomicsDB; 243140; -.
DR EnsemblPlants; AT5G24100.1; AT5G24100.1; AT5G24100.
DR GeneID; 832475; -.
DR Gramene; AT5G24100.1; AT5G24100.1; AT5G24100.
DR KEGG; ath:AT5G24100; -.
DR Araport; AT5G24100; -.
DR TAIR; locus:2178712; AT5G24100.
DR eggNOG; ENOG502QTFK; Eukaryota.
DR HOGENOM; CLU_000288_92_6_1; -.
DR InParanoid; Q9FL63; -.
DR OMA; ARTEKRY; -.
DR OrthoDB; 338975at2759; -.
DR PhylomeDB; Q9FL63; -.
DR PRO; PR:Q9FL63; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FL63; baseline and differential.
DR Genevisible; Q9FL63; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IEA:InterPro.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF00560; LRR_1; 1.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF08263; LRRNT_2; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Glycoprotein; Leucine-rich repeat; Membrane;
KW Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..614
FT /note="Inactive leucine-rich repeat receptor-like
FT serine/threonine-protein kinase At5g24100"
FT /id="PRO_0000403347"
FT TOPO_DOM 22..251
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 71..95
FT /note="LRR 1"
FT REPEAT 96..120
FT /note="LRR 2"
FT REPEAT 121..146
FT /note="LRR 3"
FT REPEAT 148..167
FT /note="LRR 4"
FT REPEAT 168..190
FT /note="LRR 5"
FT REPEAT 191..214
FT /note="LRR 6"
FT DOMAIN 341..611
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REPEAT 578..601
FT /note="LRR 7"
FT BINDING 347..355
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 369
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 343
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q94F62"
FT MOD_RES 420
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q94AG2"
FT MOD_RES 441
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q94AG2"
FT MOD_RES 514
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q94AG2"
FT MOD_RES 591
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q94AG2"
FT CARBOHYD 53
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 158
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 202
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 614 AA; 67963 MW; C61DAD9A8B3A6B74 CRC64;
MSRGRSFIFY FVLFLFFGSS ALYSQVTGDL AGDRQALLDF LNNIIHPRSL AWNTSSPVCT
TWPGVTCDID GTRVTALHLP GASLLGVIPP GTISRLSELQ ILSLRSNGLR GPFPIDFLQL
KKLKAISLGN NRFSGPLPSD YATWTNLTVL DLYSNRFNGS IPAGFANLTG LVSLNLAKNS
FSGEIPDLNL PGLRRLNFSN NNLTGSIPNS LKRFGNSAFS GNNLVFENAP PPAVVSFKEQ
KKNGIYISEP AILGIAISVC FVIFFVIAVV IIVCYVKRQR KSETEPKPDK LKLAKKMPSE
KEVSKLGKEK NIEDMEDKSE INKVMFFEGS NLAFNLEDLL IASAEFLGKG VFGMTYKAVL
EDSKVIAVKR LKDIVVSRKD FKHQMEIVGN IKHENVAPLR AYVCSKEEKL MVYDYDSNGS
LSLRLHGKNA DEGHVPLNWE TRLRFMIGVA KGLGHIHTQN LAHGNIKSSN VFMNSEGYGC
ISEAGLPLLT NPVVRADSSA RSVLRYRAPE VTDTRRSTPE SDIYSFGILM LETLTGRSIM
DDRKEGIDLV VWVNDVISKQ WTGEVFDLEL VKTPNVEAKL LQMLQLGTSC TAMVPAKRPD
MVKVVETLEE IERD
