ID   Y5435_MYCS2             Reviewed;         545 AA.
AC   A0R3D6; I7G7N1;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Putative ligase MSMEG_5435/MSMEI_5285;
DE            EC=6.2.1.-;
GN   OrderedLocusNames=MSMEG_5435, MSMEI_5285;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   PUPYLATION AT LYS-528, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20094657; DOI=10.1039/b916104j;
RA   Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA   Barry C.E. III, Bark S., Dorrestein P.C.;
RT   "Expansion of the mycobacterial 'PUPylome'.";
RL   Mol. Biosyst. 6:376-385(2010).
CC   -!- PTM: Pupylated at Lys-528 by the prokaryotic ubiquitin-like protein
CC       Pup, which probably leads to its degradation by the proteasome.
CC       {ECO:0000269|PubMed:20094657}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; CP000480; ABK72855.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP41727.1; -; Genomic_DNA.
DR   RefSeq; WP_003896832.1; NZ_SIJM01000006.1.
DR   RefSeq; YP_889674.1; NC_008596.1.
DR   AlphaFoldDB; A0R3D6; -.
DR   SMR; A0R3D6; -.
DR   STRING; 246196.MSMEI_5285; -.
DR   PRIDE; A0R3D6; -.
DR   EnsemblBacteria; ABK72855; ABK72855; MSMEG_5435.
DR   EnsemblBacteria; AFP41727; AFP41727; MSMEI_5285.
DR   GeneID; 66736738; -.
DR   KEGG; msg:MSMEI_5285; -.
DR   KEGG; msm:MSMEG_5435; -.
DR   PATRIC; fig|246196.19.peg.5296; -.
DR   eggNOG; COG0318; Bacteria.
DR   OMA; AVESNAW; -.
DR   OrthoDB; 572620at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR   CDD; cd05931; FAAL; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR028154; AMP-dep_Lig_C.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR040097; FAAL/FAAC.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF14535; AMP-binding_C_2; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Ligase; Reference proteome; Ubl conjugation.
FT   CHAIN           1..545
FT                   /note="Putative ligase MSMEG_5435/MSMEI_5285"
FT                   /id="PRO_0000395882"
FT   CROSSLNK        528
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20094657"
SQ   SEQUENCE   545 AA;  58778 MW;  6CA0EB4FD50A821D CRC64;
     MSRFTEKMYR NARTVTTGMV TGEPHEPVRH TWGEVHERAR RIAGGLAAAG IGHGDAVGVL
     AGFPVEIAPT AQGVWMRGAS LTMLHQPTPR TDLAVWAEDT MNVIGMIELK AVIISEPFLV
     ATPVLEEKGI LVLKVADLLA ADPIDPVETG EDDLALMQLT SGSTGSPKAV QITHRNIHSN
     AEAMFVGAKY DVEKDVMVSW LPCFHDMGMV GFLTIPMYFG AELVKVTPMD FLRDTLLWAK
     LIDKYKGTMT AAPNFAYALL AKRLRRQAKP GDFDLSTLRF ALSGAEPVEP ADVEDFLDAG
     KPFGLRPEAI LPAYGMAETT LAVSFSPCGE GLVVDEVDAD LLAALRRAVP ATKGNTKRLA
     SLGPLLNDLE ARVVDENGEV MPARGVGVIE LRGEPVTPGY ITMGGFVPAQ DEYGWYDTGD
     LGYITEEGNI VVCGRVKDVI IMAGRNIYPT DIERAAGRVE GVRPGCAVAV RLDAGHSRET
     FAVAVESNAW QDPAEVRRIE HQVAHEVVSE VDVRPRNVVV LGPGSIPKTP SGKLRRANSV
     SLVTQ