Y544_RICFE
ID Y544_RICFE Reviewed; 229 AA.
AC Q4UL59;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase RF_0863;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=RF_0863;
OS Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX NCBI_TaxID=315456;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC VR-1525 / URRWXCal2;
RX PubMed=15984913; DOI=10.1371/journal.pbio.0030248;
RA Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E.,
RA Parinello H., Claverie J.-M., Raoult D.;
RT "The genome sequence of Rickettsia felis identifies the first putative
RT conjugative plasmid in an obligate intracellular parasite.";
RL PLoS Biol. 3:1-12(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; CP000053; AAY61714.1; -; Genomic_DNA.
DR RefSeq; WP_011271191.1; NC_007109.1.
DR AlphaFoldDB; Q4UL59; -.
DR SMR; Q4UL59; -.
DR STRING; 315456.RF_0863; -.
DR EnsemblBacteria; AAY61714; AAY61714; RF_0863.
DR KEGG; rfe:RF_0863; -.
DR eggNOG; COG1187; Bacteria.
DR HOGENOM; CLU_024979_1_0_5; -.
DR OMA; SMEFAPF; -.
DR OrthoDB; 1037615at2; -.
DR Proteomes; UP000008548; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR Gene3D; 3.30.70.1560; -; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00093; TIGR00093; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; RNA-binding.
FT CHAIN 1..229
FT /note="Uncharacterized RNA pseudouridine synthase RF_0863"
FT /id="PRO_0000294476"
FT DOMAIN 2..69
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 102
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 229 AA; 26279 MW; E57B92AF66F82D76 CRC64;
MQRLAKIISN AGICSRRDAE KLILEGQVKV DGITILSPAT NVDISNQIEV SGILINQSQK
PRLWIYYKPV GLITTHKDPL SRKTVFEQLT GLPRVISIGR LDLNSEGLLL LTNSGDLARQ
FELPSNRLKR VYHVRAYGKS DILLKSDYKN LEIDGIFYNP HSIKLFKQNK SNSWFEVVLF
EGKNREIRRI FEYFGLKVNK LIRIQYGSFT IDNLKPGAYK EINNKILEK
