ID   Y544_RICPR              Reviewed;         235 AA.
AC   Q9ZD06;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase RP544;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=RP544;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
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DR   EMBL; AJ235272; CAA14993.1; -; Genomic_DNA.
DR   PIR; G71658; G71658.
DR   RefSeq; NP_220917.1; NC_000963.1.
DR   RefSeq; WP_004597835.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZD06; -.
DR   SMR; Q9ZD06; -.
DR   STRING; 272947.RP544; -.
DR   EnsemblBacteria; CAA14993; CAA14993; CAA14993.
DR   GeneID; 57569668; -.
DR   KEGG; rpr:RP544; -.
DR   PATRIC; fig|272947.5.peg.555; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_0_5; -.
DR   OMA; SMEFAPF; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..235
FT                   /note="Uncharacterized RNA pseudouridine synthase RP544"
FT                   /id="PRO_0000100032"
FT   DOMAIN          2..69
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        102
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   235 AA;  26815 MW;  9E09C0BAC46938A7 CRC64;
     MCRLAKIISN AGVCSRRNAE KLIVGGKVKI DGITILSPAT NVDMSNQIEV SGRLINNTQK
     PRLWIYYKPV GLITTHKDPL SRKTVFEQLI GLPRVISIGR LDLNSEGLLL LTNSGDLAHQ
     FEMPASKLKR VYNVRAYGNP NILLKNNYKN LKIDGIFYNP HSIKLLRQNK SNSWFEVVLF
     EGKNREIRRI FEYFGLQVNK LIRIQYGALK IGNLKPGNYQ EISNKILEKQ LVMCF