ID   Y548_MYCPN              Reviewed;         326 AA.
AC   P75230;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase MG370 homolog;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=MPN_548; ORFNames=G12_orf326, MP294;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; U00089; AAB95942.1; -; Genomic_DNA.
DR   PIR; S73620; S73620.
DR   RefSeq; NP_110237.1; NC_000912.1.
DR   RefSeq; WP_010874905.1; NC_000912.1.
DR   AlphaFoldDB; P75230; -.
DR   SMR; P75230; -.
DR   STRING; 272634.MPN_548; -.
DR   EnsemblBacteria; AAB95942; AAB95942; MPN_548.
DR   GeneID; 66608770; -.
DR   KEGG; mpn:MPN_548; -.
DR   PATRIC; fig|272634.6.peg.610; -.
DR   HOGENOM; CLU_016902_4_1_14; -.
DR   OMA; DRHETQF; -.
DR   BioCyc; MPNE272634:G1GJ3-903-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..326
FT                   /note="Uncharacterized RNA pseudouridine synthase MG370
FT                   homolog"
FT                   /id="PRO_0000162742"
FT   DOMAIN          15..76
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   326 AA;  37267 MW;  D87BED622FD680F3 CRC64;
     MSTAKFVVPK AVENVRIEKF CLKLLPNIKL SQFFKLLRLG KVLLNNTKAK LGQRVSSGDT
     IVFQFAIEPY LHNHSEVVDL TQVQDDLQVI FEDEQLIVVD KPAGVVCQPD AKHELFNLAN
     SLLKHCGYNQ YFKNSLSFIP RFAHRIDRNT CGLVIGAKTN QALQELEAVF RHNLLMKQYQ
     GLVFGPFNFK GTQKAYWAKD AYQALVTVKA KPFPNAKPIT TIFENSKYLT KLDLSLLTIR
     LVSGKTHQIR ACLNLLNTQL VGDRKYQLLQ FKNRNRDFKH QALHATNLSF AQLDKQKFPL
     LANYSQRQFK SQLVPWFASL IKGVSI