Y5521_DROME
ID Y5521_DROME Reviewed; 1958 AA.
AC Q9VB98; Q058W5;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Probable Rho GTPase-activating protein CG5521;
GN ORFNames=CG5521;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-789 (ISOFORM 2).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-980, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-718; SER-764; SER-767;
RP SER-787; SER-791; SER-793; SER-795; SER-1551; THR-1945 AND SER-1949, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9VB98-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9VB98-2; Sequence=VSP_037212;
CC -!- SEQUENCE CAUTION:
CC Sequence=ABJ17036.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF56644.2; -; Genomic_DNA.
DR EMBL; BT029103; ABJ17036.1; ALT_FRAME; mRNA.
DR RefSeq; NP_651516.1; NM_143259.3. [Q9VB98-1]
DR AlphaFoldDB; Q9VB98; -.
DR BioGRID; 68132; 2.
DR IntAct; Q9VB98; 6.
DR STRING; 7227.FBpp0290357; -.
DR iPTMnet; Q9VB98; -.
DR PaxDb; Q9VB98; -.
DR PRIDE; Q9VB98; -.
DR EnsemblMetazoa; FBtr0085069; FBpp0084441; FBgn0039466. [Q9VB98-1]
DR EnsemblMetazoa; FBtr0301134; FBpp0290357; FBgn0039466. [Q9VB98-2]
DR GeneID; 43242; -.
DR KEGG; dme:Dmel_CG5521; -.
DR UCSC; CG5521-RA; d. melanogaster. [Q9VB98-1]
DR FlyBase; FBgn0039466; CG5521.
DR VEuPathDB; VectorBase:FBgn0039466; -.
DR eggNOG; KOG3686; Eukaryota.
DR GeneTree; ENSGT00950000183139; -.
DR HOGENOM; CLU_001676_0_0_1; -.
DR InParanoid; Q9VB98; -.
DR OMA; FLEVIYH; -.
DR PhylomeDB; Q9VB98; -.
DR Reactome; R-DME-9013407; RHOH GTPase cycle.
DR BioGRID-ORCS; 43242; 0 hits in 3 CRISPR screens.
DR ChiTaRS; CG5521; fly.
DR GenomeRNAi; 43242; -.
DR PRO; PR:Q9VB98; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039466; Expressed in seminal fluid secreting gland and 15 other tissues.
DR ExpressionAtlas; Q9VB98; baseline and differential.
DR Genevisible; Q9VB98; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0005096; F:GTPase activator activity; ISS:FlyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IEA:InterPro.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.11210; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR035974; Rap/Ran-GAP_sf.
DR InterPro; IPR000331; Rap/Ran_GAP_dom.
DR InterPro; IPR027107; Tuberin/Ral-act_asu.
DR PANTHER; PTHR10063; PTHR10063; 1.
DR Pfam; PF02145; Rap_GAP; 1.
DR SUPFAM; SSF111347; SSF111347; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50085; RAPGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1958
FT /note="Probable Rho GTPase-activating protein CG5521"
FT /id="PRO_0000372858"
FT DOMAIN 1612..1819
FT /note="Rap-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00165"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 400..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..804
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1534..1568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1903..1958
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..417
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..703
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 764..778
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 783..804
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 718
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 764
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 767
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 787
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 791
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 793
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 795
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 980
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 1551
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1945
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1949
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 72
FT /note="K -> KELPFHI (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_037212"
SQ SEQUENCE 1958 AA; 216326 MW; 183A9FF95A587937 CRC64;
MFTKKSHADV KKSTAKLQDS KKDSASRLRH LRMILDNVEL EESKCLFETN YSHVYFILYD
TFIQAEANLK QKVHKAHREE LDGSLWLLEK ILCLLPELLA RRWQCHSLSR IMAKLLHLGN
SPKLRREGVR YFLLWYQTLG ENAPGYVHAM YADLIPGLIV PQKGVVGPDT EFSASDFLTH
PNMKADGGMA SVFHDNAFSH PVQSSEVVAL LPPSSSEKSA PPDPRDGLEV LLNSMVHTAA
CLRWRDNRAQ KDHRAFAFLL QRFMDVFLPV FSPNFDVSCS IYNPRLDLPV MRSINKKEEV
MASCVVVLIN WVSRFTHERL LSHRLDCTLH IEDVDQVRLH GYQQGLIVRD VLYVNRENIN
FVHEVYRQAF LLNFTSKPQI EAIRTAIAVY RDWMTGETPP PFLLEPNDDP PPPSNAGGTP
RSQRLRTPSY VGAIAGSKDQ LVVRAGRQNV LQVFVTNAAN VFLVNTANLN ICFPTRSRSY
RSTPLEEQTE ICKKVLNVYR TMVMNTEMDT RTWEQLLMVL LQVTSIVLHQ NQHTLPSGTN
KSATLGGILG SAIFQTLIVT WIRAHTKVPV NVLLWEKFLN VLQSLTHREE LIIEWNKTIQ
TLTRVFSRYT YGINLLDLPL DRVAESRAEK RRRIGSVWQG SGSNSAANGG SAASAAISQN
RQRESLAESS SSRSEETSSQ VPLPNHPRPH HQHTQSQGGT GHGTRPIPLT PMLSRSYSEG
SLASAARSSR IRRRRAATPK PKALQPSQLA ENRINPQDLR RAMSLDSLAR KGDAEETDSY
QEGDNESGAG SRSPSPTASS GIEGGSIKDA QLQIDASLDD ANSGSYGSGS NSGSISGRRC
IILGGSAEGW HPDSTSIMWK RMLGALGDVN RIPKADLHAQ VFMHLLEMTQ NLIKIKQNQG
ISTDNQNTQP MPPLVPPIGI VAPWCYGSLS LDRSFKKGKL WALQLLCSLA IQGAVNMQQL
PLFYHALHQL LTGEDRDLIY AILKHLEGPR LLSLLLPGHT LLLLDLVHAS AILLTSLEVS
RSTPRAEVAA LLGSLLCYPS SLLTRSVLQP TPQKFELMEC SDLQDHILNI VLRCARREPS
AKARCIALSQ LGQWLLMRLS QPLPASNSGR ANLFQQAVPH HKDVHPKASS VYNPRIKEVL
QVLLQALQFK HHTIAIVAVD SLKLCAERGR QLAAIERVPQ LIITAICKAL EIQSVTKPKD
SDKVVLTSLM LCLGEFCMAI PAPIMLTPFN EQGDTLVLQV LRVLLQVASG APRHERVKLT
ADDDFDMHIA HDDLQGDGRL PEATYQTSET IQKCITAIKL CAKAVSMHLV THIGHFPMGI
GASRLSSMVE EQDDIGNAAY GGQVETRRDS VELPSVVSAQ NMQLFMLNSG LVASFIELPT
LKLPGGGITA GLVTADKQVR VLMRDLNGKA CWDASILYSE PRNAEEPPKT TPKIQHSQPL
DSMATSMVTH TPSPRHTLRH RPAGVLPLAK DMAPDLDQLD DMLAYIGHTS PECVAPTVSQ
LNAPTASPLS GNQEAQAISV ILNQRLLEQE FVTHSTQAPS PALRHASSNS SLQQPDQRSL
HSTTASFDSL PTRTEMPFQY CRLLFSHLGL AGWERRSRTH LLQRSEKLMR ELRNVDLQKC
RETHKMAVIY VAAGQEDKGS ILRNTSGSST YEMFVSALGW EIDLETHNGF LGGLPRQGCG
ATAPYYATPF LEVVYHVATR MPSDSSEAML LKTRHLGNDE VHIVWSEHNR DYRRDILPTE
FCDVLIVVYP LRNGLFRVTV NRKPEVPWFG PLANESVVSG ACLATLIRAT AINASRTKRA
ALPLYQQFYE ERNRSLDSVS SRYKESTTFE DFASRIYNPM PLSTLGTLRE SNASSSAAPL
ASALLDHNRA SVKGWVQASI DSGPIMGIAP SASAGSTAAM EAATGMSSAS PRGPRKLGAP
FKSVTKKHSL QHIAVGGGAG AGGDTPPESP TLPQRRFK
