CASA1_SHEEP
ID CASA1_SHEEP Reviewed; 214 AA.
AC P04653; Q69B23; Q9TS03; Q9TS48;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 3.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Alpha-S1-casein;
DE Flags: Precursor;
GN Name=CSN1S1;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
RX PubMed=3002501; DOI=10.1016/s0300-9084(85)80291-1;
RA Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.;
RT "Construction and identification of recombinant plasmids carrying cDNAs
RT coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-
RT lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA.";
RL Biochimie 67:959-971(1985).
RN [2]
RP PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2),
RP PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81;
RP SER-82; SER-83; SER-90 AND SER-130, AND MASS SPECTROMETRY.
RC TISSUE=Milk;
RX PubMed=7601973; DOI=10.1017/s0022029900030983;
RA Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L.,
RA Addeo F.;
RT "Primary structure of ovine alpha s1-caseins: localization of
RT phosphorylation sites and characterization of genetic variants A, C and
RT D.";
RL J. Dairy Res. 62:281-296(1995).
RN [3]
RP VARIANT THR-209.
RC STRAIN=Comisana, Sarda, and Sopravissana; TISSUE=Blood;
RX PubMed=15328285; DOI=10.3168/jds.s0022-0302(04)73386-x;
RA Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E.,
RA Caroli A.;
RT "Single nucleotide polymorphisms in the ovine casein genes detected by
RT polymerase chain reaction-single strand conformation polymorphism.";
RL J. Dairy Sci. 87:2606-2613(2004).
CC -!- FUNCTION: Important role in the capacity of milk to transport calcium
CC phosphate.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Long;
CC IsoId=P04653-2; Sequence=Displayed;
CC Name=2; Synonyms=Short;
CC IsoId=P04653-1; Sequence=VSP_012408;
CC -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC -!- MASS SPECTROMETRY: [Isoform 1]: Mass=23638.14; Mass_error=3.0;
CC Method=Electrospray; Note=Allele A, with 11 phosphate groups. The
CC measured range is 16-214.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- MASS SPECTROMETRY: [Isoform 2]: Mass=22620.0; Mass_error=1.6;
CC Method=Electrospray; Note=Allele A, with 11 phosphate groups. The
CC measured range is 16-206.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- MASS SPECTROMETRY: [Isoform 1]: Mass=23561.8; Mass_error=2.3;
CC Method=Electrospray; Note=Allele C, with 10 phosphate groups. The
CC measured range is 16-206.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- MASS SPECTROMETRY: [Isoform 2]: Mass=22551.8; Mass_error=2.2;
CC Method=Electrospray; Note=Allele C, with 10 phosphate groups. The
CC measured range is 16-206.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- MASS SPECTROMETRY: [Isoform 1]: Mass=23290.2; Mass_error=2.9;
CC Method=Electrospray; Note=Allele D, with 6 phosphate groups. The
CC measured range is 16-214.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- MASS SPECTROMETRY: [Isoform 2]: Mass=22629.0; Mass_error=1.6;
CC Method=Electrospray; Note=Allele D, with 6 phosphate groups. The
CC measured range is 16-214.; Evidence={ECO:0000269|PubMed:7601973};
CC -!- POLYMORPHISM: At least four alleles are known. The sequence shown is
CC that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and
CC 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
CC -!- SIMILARITY: Belongs to the alpha-casein family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X03237; CAA26982.1; -; mRNA.
DR EMBL; AY444506; AAS17944.1; -; Genomic_DNA.
DR PIR; A25069; KASHS1.
DR RefSeq; NP_001009795.1; NM_001009795.1.
DR AlphaFoldDB; P04653; -.
DR STRING; 9940.ENSOARP00000011026; -.
DR Allergome; 1241; Ovi a 8.
DR Allergome; 2962; Ovi a 9.
DR iPTMnet; P04653; -.
DR PRIDE; P04653; -.
DR GeneID; 443382; -.
DR KEGG; oas:443382; -.
DR CTD; 1446; -.
DR eggNOG; ENOG502TEWT; Eukaryota.
DR Proteomes; UP000002356; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR026999; Alpha-s1_casein.
DR InterPro; IPR001588; Casein.
DR InterPro; IPR031305; Casein_CS.
DR PANTHER; PTHR10240; PTHR10240; 1.
DR Pfam; PF00363; Casein; 1.
DR PROSITE; PS00306; CASEIN_ALPHA_BETA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Milk protein;
KW Phosphoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000269|PubMed:7601973"
FT CHAIN 16..214
FT /note="Alpha-S1-casein"
FT /id="PRO_0000004459"
FT REGION 69..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..111
FT /note="Opioid-like peptide sequence"
FT COMPBIAS 74..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 27
FT /note="Phosphoserine; in allele A"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 56
FT /note="Phosphoserine; in allele C"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 63
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 79
FT /note="Phosphoserine; in alleles A and C"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 80
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 81
FT /note="Phosphoserine; in alleles A and C"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 82
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 83
FT /note="Phosphoserine; in alleles A and C"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 90
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT MOD_RES 130
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:7601973"
FT VAR_SEQ 156..163
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_012408"
FT VARIANT 28
FT /note="S -> P (in allele C and allele D)"
FT VARIANT 83
FT /note="S -> N (in allele D)"
FT VARIANT 209
FT /note="I -> T"
FT /evidence="ECO:0000269|PubMed:15328285"
SQ SEQUENCE 214 AA; 24306 MW; 9312FBFFBD6E49DC CRC64;
MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG
SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN
VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY
YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW
