ID   Y554_AQUAE              Reviewed;         238 AA.
AC   O66829;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase aq_554;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=aq_554;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000657; AAC06794.1; -; Genomic_DNA.
DR   PIR; A70350; A70350.
DR   RefSeq; NP_213389.1; NC_000918.1.
DR   RefSeq; WP_010880327.1; NC_000918.1.
DR   AlphaFoldDB; O66829; -.
DR   SMR; O66829; -.
DR   STRING; 224324.aq_554; -.
DR   PRIDE; O66829; -.
DR   EnsemblBacteria; AAC06794; AAC06794; aq_554.
DR   KEGG; aae:aq_554; -.
DR   PATRIC; fig|224324.8.peg.455; -.
DR   eggNOG; COG1187; Bacteria.
DR   HOGENOM; CLU_024979_1_2_0; -.
DR   InParanoid; O66829; -.
DR   OMA; NAHDKEY; -.
DR   OrthoDB; 1037615at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..238
FT                   /note="Uncharacterized RNA pseudouridine synthase aq_554"
FT                   /id="PRO_0000100022"
FT   DOMAIN          1..64
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        103
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   238 AA;  27862 MW;  904DDD431CD3E870 CRC64;
     MRLDKYLSKS LHISRKEAKE LIREGRVKVS GKVVKQAEYR VKEGEEVEVE GKSVKPKKNV
     YLMLYKPKGY LSTTEEDKKY PSFLELIREH FPSRKLFSAG RLDVDAEGLL LITDDGELAH
     RLTHPKWKVE KEYIVRLDRD IGDEELKKLY EVKLEEKPVQ LVKAEKLSGD TVKAILTEGR
     HHVVKRLFKA VGHNVVYLKR TRVGNLRLDE NMEPGEWREL TEEEVKELKR LVKYNPQN