Y5573_ARATH
ID Y5573_ARATH Reviewed; 892 AA.
AC Q9SI06; F4IV71;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g04300;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At2g04300; ORFNames=T23O15.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD27909.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AEC05819.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007213; AAD27909.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05819.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; ANM62529.1; -; Genomic_DNA.
DR PIR; H84455; H84455.
DR RefSeq; NP_001324680.1; NM_001335227.1.
DR RefSeq; NP_178510.1; NM_126462.1.
DR AlphaFoldDB; Q9SI06; -.
DR SMR; Q9SI06; -.
DR PaxDb; Q9SI06; -.
DR PRIDE; Q9SI06; -.
DR EnsemblPlants; AT2G04300.2; AT2G04300.2; AT2G04300.
DR GeneID; 814968; -.
DR Gramene; AT2G04300.2; AT2G04300.2; AT2G04300.
DR KEGG; ath:AT2G04300; -.
DR Araport; AT2G04300; -.
DR TAIR; locus:2059804; AT2G04300.
DR eggNOG; ENOG502QQCZ; Eukaryota.
DR InParanoid; Q9SI06; -.
DR OMA; AYENSRE; -.
DR OrthoDB; 730902at2759; -.
DR PhylomeDB; Q9SI06; -.
DR PRO; PR:Q9SI06; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SI06; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR024788; Malectin-like_Carb-bd_dom.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF12819; Malectin_like; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Glycoprotein; Kinase; Leucine-rich repeat;
KW Membrane; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome;
KW Repeat; Serine/threonine-protein kinase; Signal; Transferase;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..892
FT /note="Putative leucine-rich repeat receptor-like
FT serine/threonine-protein kinase At2g04300"
FT /id="PRO_0000403328"
FT TOPO_DOM 27..523
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..892
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 375..396
FT /note="LRR 1"
FT REPEAT 399..422
FT /note="LRR 2"
FT REPEAT 423..444
FT /note="LRR 3"
FT REPEAT 447..467
FT /note="LRR 4"
FT DOMAIN 582..855
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 707
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 588..596
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 610
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 573
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 655
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 742
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 747
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 755
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 186
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 267
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 407
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 421
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 437
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 450
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 469
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 892 AA; 99519 MW; C0AE27B3DFE2D507 CRC64;
MKTHPQAILL CVLFFITFGL LHVVEAGNQE GFISLDCGLS PNEPPYVDAA TDLTYTTDND
FVQSGKTGTI DKELESTYNK PILQLRYFPE GVRNCYTLNV TLGTNYLIRA SFVYGNYDGL
NKELEFDLYL GPNLWANVNT AVYLMNGVTT EEIIHSTKSK VLQVCLIKTG ESIPIINSLE
LRPLINDTYN TQSGSLKYLF RNYFSTSRRI IRYPNDVNDR HWYPFFDEDA WTELTTNLNV
NSSNGYDPPK FVMASASTPI SKNAPFNFTW SLIPSTAKFY SYMHFADIQT LQANETREFD
MMLNGNLALE RYRPKTFATG TIYFIKPQIC EGGQCIIELL KTSKSTLPPL CSALEVFTVI
DFPELETNQD DVIAIKNIQN TYGVSKTSWQ GDPCVPKRFM WDGLNCNNSY ISTPPTITFL
NLSSSHLTGI IASAIQNLTH LQNLDLSNNN LTGGVPEFLA GLKSLLVINL SGNNLSGSVP
QTLLQKKGLK LNLEGNIYLN CPDGSCVSKD GNGGAKKKNV VVLVVVSIAL VVVLGSALAL
FLVFRKRKTP RNEVSRTSRS LDPTITTKNR RFTYSEVVKM TNNFEKILGK GGFGMVYHGT
VNDAEQVAVK MLSPSSSQGY KEFKAEVELL LRVHHKNLVG LVGYCDEGEN LSLIYEYMAK
GDLKEHMLGN QGVSILDWKT RLKIVAESAQ GLEYLHNGCK PPMVHRDVKT TNILLDEHFQ
AKLADFGLSR SFPLEGETRV DTVVAGTPGY LDPEYYRTNW LNEKSDVYSF GIVLLEIITN
QHVINQSREK PHIAEWVGVM LTKGDIKSII DPKFSGDYDA GSVWRAVELA MSCVNPSSTG
RPTMSQVVIE LNECLASENS RRGMSQNMES KGSIQYTEVS TNFGTEYTPE AR
