Y5576_ARATH
ID Y5576_ARATH Reviewed; 579 AA.
AC Q5XF57; Q0WPA6; Q9FHH7;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Probable receptor-like serine/threonine-protein kinase At5g57670;
DE EC=2.7.11.1;
GN OrderedLocusNames=At5g57670; ORFNames=MRI1.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5XF57-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5XF57-2; Sequence=VSP_040170;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB09584.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB018118; BAB09584.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96934.1; -; Genomic_DNA.
DR EMBL; BT015759; AAU90049.1; -; mRNA.
DR EMBL; BT020463; AAW30041.1; -; mRNA.
DR EMBL; AK229173; BAF01043.1; -; mRNA.
DR RefSeq; NP_001078762.1; NM_001085293.2. [Q5XF57-1]
DR AlphaFoldDB; Q5XF57; -.
DR SMR; Q5XF57; -.
DR STRING; 3702.AT5G57670.2; -.
DR PaxDb; Q5XF57; -.
DR PRIDE; Q5XF57; -.
DR DNASU; 835873; -.
DR EnsemblPlants; AT5G57670.2; AT5G57670.2; AT5G57670. [Q5XF57-1]
DR GeneID; 835873; -.
DR Gramene; AT5G57670.2; AT5G57670.2; AT5G57670. [Q5XF57-1]
DR KEGG; ath:AT5G57670; -.
DR Araport; AT5G57670; -.
DR TAIR; locus:2172555; AT5G57670.
DR eggNOG; KOG1187; Eukaryota.
DR HOGENOM; CLU_000288_155_2_1; -.
DR InParanoid; Q5XF57; -.
DR OMA; DQKPAWR; -.
DR OrthoDB; 684563at2759; -.
DR PhylomeDB; Q5XF57; -.
DR PRO; PR:Q5XF57; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q5XF57; baseline and differential.
DR Genevisible; Q5XF57; AT.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Serine/threonine-protein kinase;
KW Transferase.
FT CHAIN 1..579
FT /note="Probable receptor-like serine/threonine-protein
FT kinase At5g57670"
FT /id="PRO_0000401360"
FT DOMAIN 267..542
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 392
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 273..281
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 295
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT MOD_RES 256
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 396
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT MOD_RES 432
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O48814"
FT VAR_SEQ 1..201
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_040170"
SQ SEQUENCE 579 AA; 65049 MW; B2F173CDE565499C CRC64;
MISCSEEGSN KILVAISLDR DESQNVLSWA INVLAKPSDT VVALHLLVGE EPRKLPMKKK
KRTQIRHAKA HVISMLGEFA YTCCHNQVNL EAKVGFSSNI GRGLIDEVKS ISAHYLVLSR
PTTHEFRIWN DIKRYVSDFA PSSCSVVLVG NQRKPHKDCY SDSAISLDIN SEKYSPRSVL
NTLSRDSLSS SGDDASSFNG SMVSSSFASP SDKPKHKSIS PYKFISSLIM NSPLRKWRGS
ETKNKPKPQP LIQCFTYNEI SKATNDFHQG NIVGIGGYSE VYRGDLWDGR RIAVKRLAKE
SGDMNKEKEF LTELGIISHV SHPNTALLLG CCVEKGLYLV FRFSENGTLY SALHENENGS
LDWPVRYKIA VGVARGLHYL HKRCNHRIIH RDIKSSNVLL GPDYEPQITD FGLAKWLPNK
WTHHAVIPVE GTFGYLAPES LMQGTIDEKT DIYAFGILLL EIITGRRPVN PTQKHILLWA
KPAMETGNTS ELVDPKLQDK YDDQQMNKLV LTASHCVQQS PILRPTMTQV LELLTNGNEA
EIAKSWRMPK DMTNDDDDNN EWDDYSMIFG YDVPSDSSF
