Y5631_BACCX
ID Y5631_BACCX Reviewed; 295 AA.
AC R8NBR0;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2013, sequence version 1.
DT 25-MAY-2022, entry version 18.
DE RecName: Full=Probable CBASS effector molecule IK1_05631 {ECO:0000305|PubMed:31533127};
GN ORFNames=IK1_05631 {ECO:0000312|EMBL:EOP43921.1};
OS Bacillus cereus (strain VD146).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1053236;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VD146;
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Feldgarden M., Van der Auwera G.A., Mahillon J., Duprez V., Timmery S.,
RA Mattelet C., Dierick K., Sun M., Yu Z., Zhu L., Hu X., Shank E.B.,
RA Swiecicka I., Hansen B.M., Andrup L., Walker B., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The genome sequence of Bacillus cereus VD146.";
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP ANTIVIRAL DEFENSE, NOMENCLATURE, AND OPERON STRUCTURE.
RC STRAIN=VD146;
RX PubMed=31533127; DOI=10.1038/s41586-019-1605-5;
RA Cohen D., Melamed S., Millman A., Shulman G., Oppenheimer-Shaanan Y.,
RA Kacen A., Doron S., Amitai G., Sorek R.;
RT "Cyclic GMP-AMP signalling protects bacteria against viral infection.";
RL Nature 574:691-695(2019).
RN [3]
RP CLASSIFICATION AND NOMENCLATURE.
RX PubMed=32839535; DOI=10.1038/s41564-020-0777-y;
RA Millman A., Melamed S., Amitai G., Sorek R.;
RT "Diversity and classification of cyclic-oligonucleotide-based anti-phage
RT signalling systems.";
RL Nat. Microbiol. 5:1608-1615(2020).
CC -!- FUNCTION: CBASS (cyclic oligonucleotide-based antiphage signaling
CC system) provides immunity against bacteriophage. The CD-NTase protein
CC synthesizes cyclic nucleotides in response to infection; these serve as
CC specific second messenger signals. The signals activate a diverse range
CC of effectors, leading to bacterial cell death and thus abortive phage
CC infection. A type I-B CBASS system (PubMed:32839535).
CC {ECO:0000269|PubMed:31533127, ECO:0000303|PubMed:32839535}.
CC -!- FUNCTION: Protects B.subtilis against phage infection. When IK1_05630
CC and IK1_05631 are introduced in B.subtilis BEST7003 there is 1000-fold
CC protection against phage SBSphiC. Both genes are required for
CC protection. Activation leads to bacterial cell lysis and death, which
CC occurs before the phage has finished its replication cycle, thus
CC protecting non-infected bacteria by aborting the phage infection and
CC preventing its propagation. {ECO:0000269|PubMed:31533127}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Part of the CBASS operon consisting of cdnD-IK1_05631.
CC {ECO:0000305|PubMed:31533127}.
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DR EMBL; AHFE01000034; EOP43921.1; -; Genomic_DNA.
DR RefSeq; WP_016119573.1; NZ_KB976676.1.
DR AlphaFoldDB; R8NBR0; -.
DR EnsemblBacteria; EOP43921; EOP43921; IK1_05631.
DR PATRIC; fig|1053236.3.peg.1318; -.
DR HOGENOM; CLU_077101_0_0_9; -.
DR Proteomes; UP000014020; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
PE 3: Inferred from homology;
KW Antiviral defense; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..295
FT /note="Probable CBASS effector molecule IK1_05631"
FT /id="PRO_0000451861"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 295 AA; 34347 MW; 1F3D66E583DE442C CRC64;
MLEKQNSEEN IELLRAMRYC YNKSKIFYAV RISISILIPI LSISIYLFNR GSTGTSNTGV
WFSVIGSIWL LIAYQIEKLE GGYIEKGAKI QEKFDINLFN IRWNNVLVGN QISPEDIRDF
SSKFKGDEEK LKNWYGGLSS KHFYVNVILA QRSNLMWAIS LKRNFSILLF TVSVLYLFLT
IAFGFFVNMS MQEYIIKILL PSMSILIYGF KTSDELKKQS NKLEALGNSI ISKFDTGNLS
EINASACREY QDAIFVYNRI RSILIPEWLY WLRQQKDDEK MIQINIRLTK KSNLF