ID   Y5656_ARATH             Reviewed;         668 AA.
AC   Q94A73; F4K0M8; Q9FJY3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=BTB/POZ domain-containing protein At5g66560;
GN   OrderedLocusNames=At5g66560; ORFNames=K1F13.23;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT   features of the regions of 1,367,185 bp covered by 19 physically assigned
RT   P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   DOMAIN BTB.
RX   PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA   Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA   Vierstra R.D.;
RT   "Cullins 3a and 3b assemble with members of the broad
RT   complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT   ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL   J. Biol. Chem. 280:18810-18821(2005).
CC   -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC       protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC       ubiquitination and subsequent proteasomal degradation of target
CC       proteins. {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC       of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC   -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00982}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB10932.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB013389; BAB10932.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED98229.1; -; Genomic_DNA.
DR   EMBL; AY049300; AAK83642.1; -; mRNA.
DR   EMBL; BT001071; AAN46836.1; -; mRNA.
DR   RefSeq; NP_201457.1; NM_126054.2.
DR   AlphaFoldDB; Q94A73; -.
DR   BioGRID; 22030; 3.
DR   IntAct; Q94A73; 3.
DR   STRING; 3702.AT5G66560.1; -.
DR   iPTMnet; Q94A73; -.
DR   PaxDb; Q94A73; -.
DR   PRIDE; Q94A73; -.
DR   ProteomicsDB; 242893; -.
DR   EnsemblPlants; AT5G66560.1; AT5G66560.1; AT5G66560.
DR   GeneID; 836788; -.
DR   Gramene; AT5G66560.1; AT5G66560.1; AT5G66560.
DR   KEGG; ath:AT5G66560; -.
DR   Araport; AT5G66560; -.
DR   TAIR; locus:2154920; AT5G66560.
DR   eggNOG; ENOG502QUA3; Eukaryota.
DR   HOGENOM; CLU_005994_6_2_1; -.
DR   InParanoid; Q94A73; -.
DR   OrthoDB; 495439at2759; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q94A73; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q94A73; differential.
DR   Genevisible; Q94A73; AT.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR043454; NPH3/RPT2-like.
DR   InterPro; IPR027356; NPH3_dom.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR32370; PTHR32370; 1.
DR   Pfam; PF03000; NPH3; 1.
DR   SMART; SM00225; BTB; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
DR   PROSITE; PS51649; NPH3; 1.
PE   2: Evidence at transcript level;
KW   Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT   CHAIN           1..668
FT                   /note="BTB/POZ domain-containing protein At5g66560"
FT                   /id="PRO_0000409587"
FT   DOMAIN          21..133
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          254..530
FT                   /note="NPH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT   REGION          73..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         471
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FMF5"
FT   CONFLICT        408
FT                   /note="D -> G (in Ref. 3; AAK83642/AAN46836)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   668 AA;  74540 MW;  BD33D23F13D8480F CRC64;
     MASEKSTSKG QAWFCTTGLP SDIEIEVDDM TFHLHKFPLM SKSRKLHRLI TEQETRSSSS
     MALITVIDPK VEETDKKGKG HEIEDDKEEE EVEEQEIEEN GYPHIKLEDF PGSSESFEMV
     AKFCYGVKMD LSASTVVPLR CAAEHLEMTE EYSPDNLISK TERFLSHSVY KSLRESIKAL
     KACESVSPLA GSLGITEQCI DSIVSRASSA DPSLFGWPVN DGGGRGNISA TDLQLIPGGA
     AKSRKKPSRD SNMELWFEDL TQLSLPIFKT VILSMRSGDL SSDIIESCLI CYAKKHIPGI
     LRSNRKPPSS SSTAVSENEQ RELLETITSN LPLDKSSISS TTRFLFGLLR TAIILNAAEI
     CRDLLERKIG SQLERATLDD LLVPSYSYLN ETLYDVDLVE RILGHFLDTL EQSNTAIVEV
     DGKSPSLMLV GKLIDGFLAE IASDANLKSD KFYNLAISLP DQARLYDDGL YRAVDVYLKA
     HPWVSEAERE KICGVMDCQK LTLEACTHAA QNERLPLRAV VQVLFFEQLQ LRHAIAGTLL
     AAQSPSTSQS TEPRPSAAIR NLTITEEDGD EAEGERQVDA GKWKKTVREN QVLRLDMDTM
     RTRVHRLERE CSNMKKVIAK IDKEGSSPAT TTDRPRSWSI TKKFGCKFKT QVCDSHEATM
     VDHRSRRS