ID   CASB_SHEEP              Reviewed;         222 AA.
AC   P11839; Q69B25;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 3.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Beta-casein;
DE   Flags: Precursor;
GN   Name=CSN2;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2505862; DOI=10.1016/0300-9084(89)90046-1;
RA   Provot C., Persuy M.A., Mercier J.-C.;
RT   "Complete nucleotide sequence of ovine beta-casein cDNA: inter-species
RT   comparison.";
RL   Biochimie 71:827-832(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7890174; DOI=10.1016/0378-1119(94)00755-h;
RA   Provot C., Persuy M.A., Mercier J.-C.;
RT   "Complete sequence of the ovine beta-casein-encoding gene and interspecies
RT   comparison.";
RL   Gene 154:259-263(1995).
RN   [3]
RP   PROTEIN SEQUENCE OF 16-222.
RX   PubMed=499202; DOI=10.1111/j.1432-1033.1979.tb13255.x;
RA   Richardson B.C., Mercier J.-C.;
RT   "The primary structure of the ovine beta-caseins.";
RL   Eur. J. Biochem. 99:285-297(1979).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-221, AND VARIANT VAL-198.
RC   STRAIN=Comisana, Sarda, and Sopravissana; TISSUE=Blood;
RX   PubMed=15328285; DOI=10.3168/jds.s0022-0302(04)73386-x;
RA   Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E.,
RA   Caroli A.;
RT   "Single nucleotide polymorphisms in the ovine casein genes detected by
RT   polymerase chain reaction-single strand conformation polymorphism.";
RL   J. Dairy Sci. 87:2606-2613(2004).
CC   -!- FUNCTION: Important role in determination of the surface properties of
CC       the casein micelles.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC   -!- POLYMORPHISM: The frequency of variant Val-198 is 0.237, 0.244 and
CC       0.176 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
CC   -!- SIMILARITY: Belongs to the beta-casein family. {ECO:0000305}.
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DR   EMBL; X16482; CAA34502.1; -; mRNA.
DR   EMBL; X79703; CAA56139.1; -; Genomic_DNA.
DR   EMBL; AY444504; AAS17942.1; -; Genomic_DNA.
DR   PIR; A32979; A32979.
DR   RefSeq; NP_001009373.1; NM_001009373.1.
DR   AlphaFoldDB; P11839; -.
DR   STRING; 9940.ENSOARP00000011245; -.
DR   Allergome; 1241; Ovi a 8.
DR   Allergome; 2964; Ovi a 11.
DR   PRIDE; P11839; -.
DR   GeneID; 443391; -.
DR   KEGG; oas:443391; -.
DR   CTD; 1447; -.
DR   eggNOG; ENOG502RU0R; Eukaryota.
DR   OrthoDB; 1336883at2759; -.
DR   Proteomes; UP000002356; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR001588; Casein.
DR   InterPro; IPR016345; Casein_beta.
DR   InterPro; IPR031305; Casein_CS.
DR   PANTHER; PTHR11500; PTHR11500; 1.
DR   Pfam; PF00363; Casein; 1.
DR   PIRSF; PIRSF002372; Beta-casein; 1.
DR   PROSITE; PS00306; CASEIN_ALPHA_BETA; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Milk protein; Phosphoprotein;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000269|PubMed:499202"
FT   CHAIN           16..222
FT                   /note="Beta-casein"
FT                   /id="PRO_0000004481"
FT   MOD_RES         27
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9GKK3"
FT   MOD_RES         30
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P05814"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P05814"
FT   MOD_RES         33
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P05814"
FT   MOD_RES         34
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P05814"
FT   VARIANT         198
FT                   /note="M -> V"
FT                   /evidence="ECO:0000269|PubMed:15328285"
FT   CONFLICT        70
FT                   /note="A -> T (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        82
FT                   /note="P -> A (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   222 AA;  24875 MW;  061B4424DCB49EB1 CRC64;
     MKVLILACLV ALALAREQEE LNVVGETVES LSSSEESITH INKKIEKFQS EEQQQTEDEL
     QDKIHPFAQA QSLVYPFTGP IPNSLPQNIL PLTQTPVVVP PFLQPEIMGV PKVKETMVPK
     HKEMPFPKYP VEPFTESQSL TLTDVEKLHL PLPLVQSWMH QPPQPLPPTV MFPPQSVLSL
     SQPKVLPVPQ KAVPQRDMPI QAFLLYQEPV LGPVRGPFPI LV