ID   Y582_THEP1              Reviewed;         567 AA.
AC   A5IK80;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=UPF0313 protein Tpet_0582 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=Tpet_0582;
OS   Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 /
OS   RKU-1).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=390874;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C.,
RA   Tapia R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Nelson K., Gogarten J.P., Noll K., Richardson P.;
RT   "Complete sequence of Thermotoga petrophila RKU-1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; CP000702; ABQ46603.1; -; Genomic_DNA.
DR   RefSeq; WP_011943201.1; NC_009486.1.
DR   AlphaFoldDB; A5IK80; -.
DR   STRING; 390874.Tpet_0582; -.
DR   EnsemblBacteria; ABQ46603; ABQ46603; Tpet_0582.
DR   KEGG; tpt:Tpet_0582; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_0; -.
DR   OMA; TCMYYTG; -.
DR   Proteomes; UP000006558; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..567
FT                   /note="UPF0313 protein Tpet_0582"
FT                   /id="PRO_1000067196"
FT   DOMAIN          288..560
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         303
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         307
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         310
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   567 AA;  64950 MW;  AF0F1E0DF0F628F7 CRC64;
     MFLPTTREEM KKLGWRELDV ILVTGDAYVD HPSFGVALIG HYLVSHGFKV GIIAQPDWRT
     EKDITRLGRP RLFFGVTAGN VDSMVANYTA SKKKRKTDDY TPGGSGGKRP DRATIVYTNL
     IKRFFPEVPV VLGGLEASLR RFAHYDWWSE KVRKSILIDS KVDLLVYGMG EKAVLEIAQI
     LSRTGDIEKC KSVRGVVWWS SQKPEGGIEL PSYDEISENP EKYAEALKLQ TWYTDPYKNI
     LIYQKQDTRY VVQNPPQLPL SQEELDRLYL LPFEREVHPF YAKMGRVKAI ETVKFSITAV
     RGCFGSCSFC ALTQHQTTHV SYRSKDSILE EVRILTKKKD FKGTITDVGG PTANLYGSSC
     SIRETKGQCQ KFCLYPSVCK IVRPNHDEFI SLLESIRKIP GVRNVFVSSG IRHDFVLAEK
     DPDVFIRELV KYTPGQLKLA PEHAHPKVLS LMRKPPVELF LEFKRRFETL AKKIGKRKYV
     IGYFIVGHPG EGWRENNYLR DFILKHLGYF PQQIQIFTPT PGTVSTAMYY SGLDPFTGEK
     VHVERSLKVR NKMKENVLFK KKGREKR