Y5880_ARATH
ID Y5880_ARATH Reviewed; 614 AA.
AC Q9FKB6; Q8GZ23;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=BTB/POZ domain-containing protein At5g48800;
GN OrderedLocusNames=At5g48800; ORFNames=K24G6.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP DOMAIN BTB.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FKB6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FKB6-2; Sequence=VSP_030534, VSP_030535;
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
CC -!- SIMILARITY: Belongs to the NPH3 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00982}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX830207; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AB012242; BAB09433.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95726.1; -; Genomic_DNA.
DR EMBL; AK117251; BAC41926.1; -; mRNA.
DR EMBL; BX830207; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_199691.1; NM_124257.4. [Q9FKB6-1]
DR AlphaFoldDB; Q9FKB6; -.
DR SMR; Q9FKB6; -.
DR iPTMnet; Q9FKB6; -.
DR PaxDb; Q9FKB6; -.
DR PRIDE; Q9FKB6; -.
DR ProteomicsDB; 242881; -. [Q9FKB6-1]
DR EnsemblPlants; AT5G48800.1; AT5G48800.1; AT5G48800. [Q9FKB6-1]
DR GeneID; 834938; -.
DR Gramene; AT5G48800.1; AT5G48800.1; AT5G48800. [Q9FKB6-1]
DR KEGG; ath:AT5G48800; -.
DR Araport; AT5G48800; -.
DR TAIR; locus:2156524; AT5G48800.
DR eggNOG; ENOG502R4F9; Eukaryota.
DR HOGENOM; CLU_005994_6_0_1; -.
DR InParanoid; Q9FKB6; -.
DR OMA; SHRTDSK; -.
DR PhylomeDB; Q9FKB6; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9FKB6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKB6; baseline and differential.
DR Genevisible; Q9FKB6; AT.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043454; NPH3/RPT2-like.
DR InterPro; IPR027356; NPH3_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR32370; PTHR32370; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF03000; NPH3; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
DR PROSITE; PS51649; NPH3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..614
FT /note="BTB/POZ domain-containing protein At5g48800"
FT /id="PRO_0000315353"
FT DOMAIN 43..111
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 219..484
FT /note="NPH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00982"
FT REGION 492..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 583..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 522..562
FT /evidence="ECO:0000255"
FT COMPBIAS 583..600
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 425
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9FMF5"
FT VAR_SEQ 316..321
FT /note="LGSQLD -> RHFVR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_030534"
FT VAR_SEQ 322..614
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_030535"
SQ SEQUENCE 614 AA; 69549 MW; 6C2C65FB416D6095 CRC64;
MDKHHHQHLL LQHHQHLHHH QKLSLAKSSR QSCSEWIFRD VPSDITIEVN GGNFALHKFP
LVSRSGRIRR IVAEHRDSDI SKVELLNLPG GAETFELAAK FCYGINFEIT SSNVAQLFCV
SDYLEMTEEY SKDNLASRTE EYLESIVCKN LEMCVQVLKQ SEILLPLADE LNIIGRCIDA
IASKACAEQI ASSFSRLEYS SSGRLHMSRQ VKSSGDGGDW WIEDLSVLRI DLYQRVMNAM
KCRGVRPESI GASLVSYAER ELTKRSEHEQ TIVETIVTLL PVENLVVPIS FLFGLLRRAV
ILDTSVSCRL DLERRLGSQL DMATLDDLLI PSFRHAGDTL FDIDTVHRIL VNFSQQGGDD
SEDEESVFEC DSPHSPSQTA MFKVAKLVDS YLAEIAPDAN LDLSKFLLIA EALPPHARTL
HDGLYRAIDL YLKAHQGLSD SDKKKLSKLI DFQKLSQEAG AHAAQNERLP LQSIVQVLYF
EQLKLRSSLC SSYSDEEPKP KQQQQQSWRI NSGALSATMS PKDNYASLRR ENRELKLELA
RLRMRLNDLE KEHICMKRDM QRSHSRKFMS SFSKKMGKLS FFGHSSSRGS SSPSKQSFRT
DSKVLMERTC ASTD
