ID   Y593_MYCLE              Reviewed;         869 AA.
AC   Q49689; O33141;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=UPF0051 protein ML0593;
DE   Contains:
DE     RecName: Full=Mle pps1 intein;
GN   OrderedLocusNames=ML0593; ORFNames=B1496_C2_189, MLCL536.28c;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the UPF0051 (ycf24) family. {ECO:0000305}.
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DR   EMBL; U00013; AAA17127.1; -; Genomic_DNA.
DR   EMBL; Z99125; CAB16171.1; -; Genomic_DNA.
DR   EMBL; Z99125; CAB16172.1; -; Genomic_DNA.
DR   EMBL; AL583919; CAC30101.1; -; Genomic_DNA.
DR   PIR; A86983; A86983.
DR   PIR; S72760; S72760.
DR   RefSeq; NP_301502.1; NC_002677.1.
DR   RefSeq; WP_010907826.1; NC_002677.1.
DR   AlphaFoldDB; Q49689; -.
DR   SMR; Q49689; -.
DR   STRING; 272631.ML0593; -.
DR   EnsemblBacteria; CAC30101; CAC30101; CAC30101.
DR   KEGG; mle:ML0593; -.
DR   PATRIC; fig|272631.5.peg.1031; -.
DR   Leproma; ML0593; -.
DR   eggNOG; COG0719; Bacteria.
DR   eggNOG; COG1372; Bacteria.
DR   HOGENOM; CLU_007402_0_0_11; -.
DR   OMA; NERNGWK; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR010231; SUF_FeS_clus_asmbl_SufB.
DR   InterPro; IPR000825; SUF_FeS_clus_asmbl_SufBD.
DR   InterPro; IPR037284; SUF_FeS_clus_asmbl_SufBD_sf.
DR   Pfam; PF14528; LAGLIDADG_3; 1.
DR   Pfam; PF01458; SUFBD; 1.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SUPFAM; SSF101960; SSF101960; 2.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01980; sufB; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   3: Inferred from homology;
KW   Autocatalytic cleavage; Protein splicing; Reference proteome.
FT   CHAIN           1..201
FT                   /note="UPF0051 protein ML0593, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036187"
FT   CHAIN           202..587
FT                   /note="Mle pps1 intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036188"
FT   CHAIN           588..869
FT                   /note="UPF0051 protein ML0593, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000036189"
FT   DOMAIN          344..477
FT                   /note="DOD-type homing endonuclease"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT   CONFLICT        482
FT                   /note="A -> R (in Ref. 1; AAA17127)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   869 AA;  95573 MW;  DB04CF70CB50765A CRC64;
     MTRTSETTKS PAPELLTQQQ AIDSLGKYGY GWADSDVAGA SARRGLSEDV VRDISAKKDE
     PEWMLQARLK ALRVFERKPM PRWGSNLDGI DFDNIKYFVR STEKQAASWD ELPEDIRNTY
     DRLGIPDAEK QRLVAGVAAQ YESEVVYHQI RADLKDQGVV FLDTETGLRE YPDIFKQYLG
     TVIPAGDNKF SALNTAVWSG GCLTADARIN VKGKGLVSIA DVQPGDEVFG VNIGCELERG
     KVLAKVASGT KPVYEMHVAG RALEATGNHQ FLVARRVEEG KRTRWTAVWA PLEEIESGEP
     IAVARVLPDD SGTIFFSESE LDIKNRTRQC LYFPCQNSVD LLWLLGLWLG DGHTAAPHKH
     MRQVAFSVPA GDPVHHTAIR VVSEQFGANV TVVNCGFIVS SKAFETWLAE LGFSGDEKTK
     RLPAWIYSLP HEHQLALIGG LVDADGWTES SGATMSIAFA SRELLEDVRQ LAIGCGLYPD
     GALVERTRSA TCRDGRIVTS TSWRLRIQGS LDRVGTRTPG KRGKPVSNKG RRQRYVAAAG
     LNFSSLSTDT VGFARLKSKT LVGEKPTYDI QVVGLENFVA NGIVAHNSFI YVPPGVHVDI
     PLQAYFRINT ENMGQFERTL IIADTGSYVH YVEGCTAPIY KSDSLHSAVV EIIVKPHARV
     RYTTIQNWSN NVYNLVTKRA RVETGATMEW IDGNIGSKVT MKYPAVWMTG EHAKGEVLSV
     AFAGEGQHQD TGAKMLHLAS NTSSNIVSKS VARGGGRTSY RGLVQVNKGA HGSRSSVKCD
     ALLVDTISRS DTYPYVDIRE DDVTMGHEAT VSKVSENQLF YLMSRGLAED EAMAMVVRGF
     VEPIAKELPM EYALELNRLI ELQMEGAVG