ID   Y602_GEOSW              Reviewed;         176 AA.
AC   C5D6I2;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Putative metal-dependent hydrolase GWCH70_0602 {ECO:0000255|HAMAP-Rule:MF_01256};
DE            EC=3.-.-.- {ECO:0000255|HAMAP-Rule:MF_01256};
GN   OrderedLocusNames=GWCH70_0602;
OS   Geobacillus sp. (strain WCH70).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   unclassified Geobacillus.
OX   NCBI_TaxID=471223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WCH70;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Brumm P., Mead D.A., Richardson P.;
RT   "Complete sequence of chromosome of Geopacillus sp. WCH70.";
RL   Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possible metal-dependent hydrolase. {ECO:0000255|HAMAP-
CC       Rule:MF_01256}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01256};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01256};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SIMILARITY: Belongs to the metal hydrolase YfiT family.
CC       {ECO:0000255|HAMAP-Rule:MF_01256}.
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DR   EMBL; CP001638; ACS23499.1; -; Genomic_DNA.
DR   RefSeq; WP_012749303.1; NC_012793.1.
DR   AlphaFoldDB; C5D6I2; -.
DR   SMR; C5D6I2; -.
DR   STRING; 471223.GWCH70_0602; -.
DR   EnsemblBacteria; ACS23499; ACS23499; GWCH70_0602.
DR   KEGG; gwc:GWCH70_0602; -.
DR   eggNOG; COG2318; Bacteria.
DR   HOGENOM; CLU_105789_1_0_9; -.
DR   OMA; GWTIRQV; -.
DR   OrthoDB; 1812981at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.450; -; 1.
DR   HAMAP; MF_01256; YfiT_hydrol; 1.
DR   InterPro; IPR024775; DinB-like.
DR   InterPro; IPR034660; DinB/YfiT-like.
DR   InterPro; IPR023774; Put_metal_dep_hydrolase_YfiT.
DR   Pfam; PF12867; DinB_2; 1.
DR   SUPFAM; SSF109854; SSF109854; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Metal-binding; Zinc.
FT   CHAIN           1..176
FT                   /note="Putative metal-dependent hydrolase GWCH70_0602"
FT                   /id="PRO_1000214120"
FT   BINDING         65
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         158
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         162
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
SQ   SEQUENCE   176 AA;  20429 MW;  27D7CEC5D6A88FF1 CRC64;
     MDEIRYPIGK FQAPAAFCEE DAKQWISDIR QIPAKLWEAV SGLNEEQLNT PYRDGGWTVA
     QVVHHIADAS MNAFLRTKWT LTEDVPSIKP FEENDWAKTT EARSLHIEPS LQLLEGLHER
     WAHLLESMSS DDFQRNFYHE GTKENVPLYV LIAMHAWHGK HHTAQIVSLR QRKGWN