CASK_MOUSE
ID CASK_MOUSE Reviewed; 181 AA.
AC P06796; Q9D1U2;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Kappa-casein;
DE Flags: Precursor;
GN Name=Csn3; Synonyms=Csn10, Csnk;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=4042811; DOI=10.1089/dna.1985.4.263;
RA Thompson M.D., Dave J.R., Nakhasi H.L.;
RT "Molecular cloning of mouse mammary gland kappa-casein: comparison with rat
RT kappa-casein and rat and human gamma-fibrinogen.";
RL DNA 4:263-271(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Kappa-casein stabilizes micelle formation, preventing casein
CC precipitation in milk.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC -!- SIMILARITY: Belongs to the kappa-casein family. {ECO:0000305}.
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DR EMBL; M10114; AAA37370.1; -; mRNA.
DR EMBL; AK021350; BAB32382.1; -; mRNA.
DR EMBL; AK085710; BAC39516.1; -; mRNA.
DR EMBL; AK142607; BAE25129.1; -; mRNA.
DR EMBL; AK142673; BAE25155.1; -; mRNA.
DR EMBL; AK164788; BAE37916.1; -; mRNA.
DR EMBL; AC022298; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC049949; AAH49949.1; -; mRNA.
DR EMBL; BC050269; AAH50269.1; -; mRNA.
DR CCDS; CCDS19396.1; -.
DR PIR; A05080; A05080.
DR RefSeq; NP_031812.2; NM_007786.4.
DR RefSeq; XP_006534828.1; XM_006534765.2.
DR AlphaFoldDB; P06796; -.
DR BioGRID; 198941; 1.
DR STRING; 10090.ENSMUSP00000108896; -.
DR GlyGen; P06796; 4 sites.
DR PhosphoSitePlus; P06796; -.
DR CPTAC; non-CPTAC-3317; -.
DR PaxDb; P06796; -.
DR PeptideAtlas; P06796; -.
DR PRIDE; P06796; -.
DR Antibodypedia; 24320; 100 antibodies from 21 providers.
DR DNASU; 12994; -.
DR Ensembl; ENSMUST00000001667; ENSMUSP00000001667; ENSMUSG00000001622.
DR Ensembl; ENSMUST00000113267; ENSMUSP00000108892; ENSMUSG00000001622.
DR Ensembl; ENSMUST00000113271; ENSMUSP00000108896; ENSMUSG00000001622.
DR GeneID; 12994; -.
DR KEGG; mmu:12994; -.
DR UCSC; uc008xzg.1; mouse.
DR CTD; 1448; -.
DR MGI; MGI:107461; Csn3.
DR VEuPathDB; HostDB:ENSMUSG00000001622; -.
DR eggNOG; ENOG502TM2T; Eukaryota.
DR GeneTree; ENSGT00390000009184; -.
DR HOGENOM; CLU_103388_0_0_1; -.
DR InParanoid; P06796; -.
DR OMA; HPSFIAI; -.
DR OrthoDB; 1540917at2759; -.
DR TreeFam; TF338369; -.
DR Reactome; R-MMU-5223345; Miscellaneous transport and binding events.
DR BioGRID-ORCS; 12994; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Csn3; mouse.
DR PRO; PR:P06796; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P06796; protein.
DR Bgee; ENSMUSG00000001622; Expressed in thoracic mammary gland and 62 other tissues.
DR Genevisible; P06796; MM.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0007595; P:lactation; IMP:MGI.
DR GO; GO:0050821; P:protein stabilization; ISO:MGI.
DR InterPro; IPR000117; Casein_kappa.
DR PANTHER; PTHR11470; PTHR11470; 1.
DR Pfam; PF00997; Casein_kappa; 1.
DR PIRSF; PIRSF002374; Casein_kappa; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Milk protein; Phosphoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000250"
FT CHAIN 22..181
FT /note="Kappa-casein"
FT /id="PRO_0000004499"
FT SITE 118..119
FT /note="Cleavage; by chymosin/rennin"
FT MOD_RES 123
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 162
FT /note="Phosphoserine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT MOD_RES 178
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02670"
FT CARBOHYD 134
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 144
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 155
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 162
FT /note="O-linked (GalNAc...) serine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CONFLICT 9
FT /note="V -> M (in Ref. 1; AAA37370)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 181 AA; 20030 MW; 0EBEBF116A147F66 CRC64;
MMRNFIVVVN ILALTLPFLA AEIQNPDSNC RGEKNDIVYD EQRVLYTPVR SVLNFNQYEP
NYYHYRPSLP ATASPYMYYP LVVRLLLLRS PAPISKWQSM PNFPQSAGVP YAIPNPSFLA
MPTNENQDNT AIPTIDPITP IVSTPVPTME SIVNTVANPE ASTVSINTPE TTTVPVSSTA
A
