ID   Y6031_MYCS2             Reviewed;         279 AA.
AC   A0R518; I7FLX4;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Putative short-chain type dehydrogenase/reductase MSMEG_6031/MSMEI_5872;
DE            EC=1.1.1.-;
GN   OrderedLocusNames=MSMEG_6031, MSMEI_5872;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   PUPYLATION AT LYS-65, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20094657; DOI=10.1039/b916104j;
RA   Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA   Barry C.E. III, Bark S., Dorrestein P.C.;
RT   "Expansion of the mycobacterial 'PUPylome'.";
RL   Mol. Biosyst. 6:376-385(2010).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK70855.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42305.1; -; Genomic_DNA.
DR   RefSeq; WP_011730987.1; NZ_SIJM01000017.1.
DR   RefSeq; YP_890256.1; NC_008596.1.
DR   AlphaFoldDB; A0R518; -.
DR   SMR; A0R518; -.
DR   STRING; 246196.MSMEI_5872; -.
DR   EnsemblBacteria; ABK70855; ABK70855; MSMEG_6031.
DR   EnsemblBacteria; AFP42305; AFP42305; MSMEI_5872.
DR   GeneID; 66737317; -.
DR   KEGG; msg:MSMEI_5872; -.
DR   KEGG; msm:MSMEG_6031; -.
DR   PATRIC; fig|246196.19.peg.5869; -.
DR   eggNOG; COG1028; Bacteria.
DR   OMA; VGWVEPV; -.
DR   OrthoDB; 1356861at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   InterPro; IPR023985; SDR_subfam_1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR03971; SDR_subfam_1; 1.
PE   1: Evidence at protein level;
KW   Isopeptide bond; NAD; Oxidoreductase; Reference proteome; Ubl conjugation.
FT   CHAIN           1..279
FT                   /note="Putative short-chain type dehydrogenase/reductase
FT                   MSMEG_6031/MSMEI_5872"
FT                   /id="PRO_0000396811"
FT   ACT_SITE        171
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         11..33
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         158
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CROSSLNK        65
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20094657"
SQ   SEQUENCE   279 AA;  29442 MW;  A5AAFFFF4200DAA7 CRC64;
     MAGRVEGKVA FITGAARGQG RSHAVRLAEE GADIIAVDVC RRISSNEDIP ASTPEDLAET
     VELVKGLNRR IVAEEVDVRD YDALKAVVDS GVEQLGGLDI VVANAGIGNG GATLDKTSEA
     DWDDMIGVNL SGVWKTVKAA VPHLISGGNG GSIILTSSVG GLKAYPHTGH YIAAKHGVVG
     LMRTFAVELG QHSIRVNSVH PTNVNTPLFM NEGTMKLFRP DLENPGPDDM AVVAQMMHVL
     PVGWVEPRDI SNAVLFLASD EARYVTGLPM TVDAGSMLK