ID   Y6073_MYCS2             Reviewed;         314 AA.
AC   A0R557; I7GFD3;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=MSMEG_6073, MSMEI_5913;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. {ECO:0000305}.
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DR   EMBL; CP000480; ABK75506.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42346.1; -; Genomic_DNA.
DR   RefSeq; WP_011731016.1; NZ_SIJM01000046.1.
DR   RefSeq; YP_890295.1; NC_008596.1.
DR   AlphaFoldDB; A0R557; -.
DR   SMR; A0R557; -.
DR   STRING; 246196.MSMEI_5913; -.
DR   PRIDE; A0R557; -.
DR   EnsemblBacteria; ABK75506; ABK75506; MSMEG_6073.
DR   EnsemblBacteria; AFP42346; AFP42346; MSMEI_5913.
DR   GeneID; 66737357; -.
DR   KEGG; msg:MSMEI_5913; -.
DR   KEGG; msm:MSMEG_6073; -.
DR   PATRIC; fig|246196.19.peg.5911; -.
DR   eggNOG; COG0566; Bacteria.
DR   OMA; QVPPYEY; -.
DR   OrthoDB; 1422015at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR013123; SpoU_subst-bd.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR46429; PTHR46429; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   Pfam; PF08032; SpoU_sub_bind; 1.
DR   SMART; SM00967; SpoU_sub_bind; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
DR   TIGRFAMs; TIGR00186; rRNA_methyl_3; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..314
FT                   /note="Uncharacterized tRNA/rRNA methyltransferase
FT                   MSMEG_6073/MSMEI_5913"
FT                   /id="PRO_0000379577"
FT   REGION          1..71
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         266
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         286
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         295
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  32572 MW;  590442B8CFC5323D CRC64;
     MAGNSQRRGA VRKPGTKKGP TVGSGGVRRR GLEGRGATPP AHMRPNHPAA KKARAAAKAQ
     QQRAGRKADE TELVLGRNPV LECLRAKVPA TALYVALGTD ADERVTESVQ IAADRGISIL
     EVPRTDLDRM SNHGLHQGLG LQVPPYAYAH PDDLLATARA DAAPALLVAL DNISDPRNLG
     AIVRSVAAFA GHGVVIPQRR SASVTAVAWR TSAGAAARVP VARATNLNRT LKSFADAGLQ
     IVGLDAGGDT TLDELDATGP TVVVVGSEGK GLSRLVREHC DAIVSIPMAG PTESLNASVA
     AGVVLAEIAR QRRS