ID   Y612_SYNY3              Reviewed;         261 AA.
AC   P72581;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase slr0612;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=slr0612;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA16580.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; BA000022; BAA16580.1; ALT_FRAME; Genomic_DNA.
DR   PIR; S74428; S74428.
DR   STRING; 1148.1651652; -.
DR   PaxDb; P72581; -.
DR   EnsemblBacteria; BAA16580; BAA16580; BAA16580.
DR   KEGG; syn:slr0612; -.
DR   eggNOG; COG1187; Bacteria.
DR   InParanoid; P72581; -.
DR   PhylomeDB; P72581; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0140098; F:catalytic activity, acting on RNA; IEA:UniProt.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   Gene3D; 3.30.70.1560; -; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR   InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00093; TIGR00093; 1.
DR   PROSITE; PS01149; PSI_RSU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..261
FT                   /note="Uncharacterized RNA pseudouridine synthase slr0612"
FT                   /id="PRO_0000100033"
FT   ACT_SITE        81
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   261 AA;  29562 MW;  601A453085C04A69 CRC64;
     MSAPRESLLE LVDYVLGRLX LGFPVDFFPA GLIALNKTPQ TIVFYKPYGV LCQFTDNSAH
     PRPTLKDYIN LPDLYPVGRL DQDSEGLLLL TSNGKLQHRL AHREFAHQRT YFAQVEGSPT
     DEDLEPLRRG ITFADYPTRP AIAKIITEPD FPPRNPPIRY RASIPTSWLS ITLTEGRNRQ
     VRRMTAAVGF PTLRLVRVQI QVTGRSPQQG KGKSAATWCL TLEGLSPGQW RPLTPWEENF
     CQQLLTGNPN GPWQKKFGDR R