ID   Y613_STRA3              Reviewed;         451 AA.
AC   Q8E6F5;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Uncharacterized RNA methyltransferase gbs0613;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=gbs0613;
OS   Streptococcus agalactiae serotype III (strain NEM316).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=211110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEM316;
RX   PubMed=12354221; DOI=10.1046/j.1365-2958.2002.03126.x;
RA   Glaser P., Rusniok C., Buchrieser C., Chevalier F., Frangeul L., Msadek T.,
RA   Zouine M., Couve E., Lalioui L., Poyart C., Trieu-Cuot P., Kunst F.;
RT   "Genome sequence of Streptococcus agalactiae, a pathogen causing invasive
RT   neonatal disease.";
RL   Mol. Microbiol. 45:1499-1513(2002).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AL766846; CAD46257.1; -; Genomic_DNA.
DR   RefSeq; WP_000902654.1; NC_004368.1.
DR   AlphaFoldDB; Q8E6F5; -.
DR   SMR; Q8E6F5; -.
DR   STRING; 211110.gbs0613; -.
DR   EnsemblBacteria; CAD46257; CAD46257; CAD46257.
DR   GeneID; 66885546; -.
DR   KEGG; san:gbs0613; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000000823; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..451
FT                   /note="Uncharacterized RNA methyltransferase gbs0613"
FT                   /id="PRO_0000162024"
FT   DOMAIN          1..59
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        408
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         283
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         312
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         333
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         381
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   451 AA;  50993 MW;  8404BFC52AEF2F4C CRC64;
     MLHKNDIIET EISDISHEGM GIAKVDGFVF FVENALPGEI IKMRVLKLRK RIGYGKVEEY
     LTTSPHRNEG LDYTYLRTGI ADLGHLTYEQ QLLFKQKQVA DNLYKIAHIS DVLVEPTLGM
     TIPLAYRNKA QVPVRRVDGQ LETGFFRKNS HTLVSIEDYL IQEKEIDALI NFTRDLLRKF
     DVKPYDEEQQ SGLIRNLVVR RGHYTGQLML VLVTTRPKIF RIDQMIEKLV SAFPSVVSIM
     QNINDRNSNV IFGKEFRTLY GSDTIEDQML GNTYAISAQS FYQVNTEMAE KLYQKAIDFS
     DLNSEDIVID AYSGIGTIGL SVAKQVKHVY GVEVVEKAVS DAKENATRNG ITNSTYVADS
     AENAMAKWLK EGIKPTVIMV DPPRKGLTES FVYSAAQTKA DKITYISCNS ATMARDIKLF
     EELGYHLVKI QPVDLFPMTH HVECVALLVK A