ID   Y616_GEOKA              Reviewed;         178 AA.
AC   Q5L2C9;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Putative metal-dependent hydrolase GK0616 {ECO:0000255|HAMAP-Rule:MF_01256};
DE            EC=3.-.-.- {ECO:0000255|HAMAP-Rule:MF_01256};
GN   OrderedLocusNames=GK0616;
OS   Geobacillus kaustophilus (strain HTA426).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC   Geobacillus thermoleovorans group.
OX   NCBI_TaxID=235909;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HTA426;
RX   PubMed=15576355; DOI=10.1093/nar/gkh970;
RA   Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA   Matsui S., Uchiyama I.;
RT   "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT   Geobacillus kaustophilus.";
RL   Nucleic Acids Res. 32:6292-6303(2004).
CC   -!- FUNCTION: Possible metal-dependent hydrolase. {ECO:0000255|HAMAP-
CC       Rule:MF_01256}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01256};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01256};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   -!- SIMILARITY: Belongs to the metal hydrolase YfiT family.
CC       {ECO:0000255|HAMAP-Rule:MF_01256}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000043; BAD74901.1; -; Genomic_DNA.
DR   RefSeq; WP_011230120.1; NC_006510.1.
DR   AlphaFoldDB; Q5L2C9; -.
DR   SMR; Q5L2C9; -.
DR   STRING; 235909.GK0616; -.
DR   EnsemblBacteria; BAD74901; BAD74901; GK0616.
DR   KEGG; gka:GK0616; -.
DR   eggNOG; COG2318; Bacteria.
DR   HOGENOM; CLU_105789_1_0_9; -.
DR   OMA; GWTIRQV; -.
DR   Proteomes; UP000001172; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.450; -; 1.
DR   HAMAP; MF_01256; YfiT_hydrol; 1.
DR   InterPro; IPR024775; DinB-like.
DR   InterPro; IPR034660; DinB/YfiT-like.
DR   InterPro; IPR023774; Put_metal_dep_hydrolase_YfiT.
DR   Pfam; PF12867; DinB_2; 1.
DR   SUPFAM; SSF109854; SSF109854; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..178
FT                   /note="Putative metal-dependent hydrolase GK0616"
FT                   /id="PRO_0000162376"
FT   BINDING         68
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         161
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
FT   BINDING         165
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01256"
SQ   SEQUENCE   178 AA;  19713 MW;  0DEE4D777B701969 CRC64;
     MATVDPIRYP IGTFQAPQQF EAGEVQEWIA AIRGLPSDLR TAVSGLNDEQ LNTPYREGGW
     TVAQVVHHLA DASMNAFLRT KWGVTEDGPT VKPFAESEWA KTADACLLPI EPSLLLLDGL
     HARWAALLES MTEADFHRTV RPEGAAGEMP LYVLTALYAW HGKHHTAQVA SLRKRKGW