CASK_SHEEP
ID CASK_SHEEP Reviewed; 192 AA.
AC P02669; Q69B24; Q6JZK3;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Kappa-casein;
DE Flags: Precursor;
GN Name=CSN3; Synonyms=CSN10, CSNK;
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2402453; DOI=10.1093/nar/18.17.5286;
RA Furet J.-P., Mercier J.-C., Soulier S., Gaye P., Hue-Delahaie D.,
RA Vilotte J.-L.;
RT "Nucleotide sequence of ovine kappa-casein cDNA.";
RL Nucleic Acids Res. 18:5286-5286(1990).
RN [2]
RP PROTEIN SEQUENCE OF 22-129 (VARIANT A).
RX PubMed=4605338; DOI=10.1111/j.1432-1033.1974.tb03604.x;
RA Jolles J., Schoentgen F., Hermann J., Alais C., Jolles P.;
RT "The sequence of sheep kappa-casein: primary structure of para-kappa A-
RT casein.";
RL Eur. J. Biochem. 46:127-132(1974).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-192.
RA Feligini M., Curik V.C., Parma P., Greppi G.F., Enne G.;
RT "A single nucleotide polymorphism in the sheep K-casein (CSN3) coding
RT region.";
RL (In) van der Honing Y. (eds.);
RL The 54th Annual meeting of the European Association for Animal Production
RL (EAAP), Rome, Italy, Sep. 2003, pp.100-100, Wageningen Academic,
RL Netherlands (2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-187, AND VARIANT LEU-125.
RC STRAIN=Comisana, Sarda, and Sopravissana; TISSUE=Blood;
RX PubMed=15328285; DOI=10.3168/jds.s0022-0302(04)73386-x;
RA Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E.,
RA Caroli A.;
RT "Single nucleotide polymorphisms in the ovine casein genes detected by
RT polymerase chain reaction-single strand conformation polymorphism.";
RL J. Dairy Sci. 87:2606-2613(2004).
RN [5]
RP PROTEIN SEQUENCE OF 127-192 (VARIANT A).
RX PubMed=4429673;
RA Jolles J., Fiat A.-M., Schoentgen F., Alais C., Jolles P.;
RT "The amino acid sequence of sheep kappa A-casein. II. Sequence studies
RT concerning the kappa A-caseinoglycopeptide and establishment of the
RT complete primary structure of the protein.";
RL Biochim. Biophys. Acta 365:335-343(1974).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-192.
RA Woollard J.R., Dentine M.R.;
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Kappa-casein stabilizes micelle formation, preventing casein
CC precipitation in milk.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
CC -!- PTM: O-glycosylated on Thr at position 156, 158 or 159.
CC -!- POLYMORPHISM: The frequency of variant Leu-125 is 0.015 in the Italian
CC breed Sopravissana.
CC -!- SIMILARITY: Belongs to the kappa-casein family. {ECO:0000305}.
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DR EMBL; X51822; CAA36122.1; -; mRNA.
DR EMBL; AY237637; AAP69943.1; -; Genomic_DNA.
DR EMBL; AY444505; AAS17943.1; -; Genomic_DNA.
DR EMBL; U84256; AAB47262.1; -; Genomic_DNA.
DR PIR; S14711; KKSHA.
DR RefSeq; NP_001009378.1; NM_001009378.1.
DR AlphaFoldDB; P02669; -.
DR STRING; 9940.ENSOARP00000011880; -.
DR Allergome; 1241; Ovi a 8.
DR PRIDE; P02669; -.
DR Ensembl; ENSOART00000012054; ENSOARP00000011880; ENSOARG00000011084.
DR Ensembl; ENSOART00020001919; ENSOARP00020001592; ENSOARG00020001292.
DR GeneID; 443394; -.
DR KEGG; oas:443394; -.
DR CTD; 1448; -.
DR eggNOG; ENOG502TM2T; Eukaryota.
DR HOGENOM; CLU_103388_0_0_1; -.
DR OMA; HPSFIAI; -.
DR OrthoDB; 1540917at2759; -.
DR Proteomes; UP000002356; Chromosome 6.
DR Bgee; ENSOARG00000011084; Expressed in mammary gland and 12 other tissues.
DR ExpressionAtlas; P02669; differential.
DR GO; GO:0005615; C:extracellular space; IEA:Ensembl.
DR GO; GO:0007595; P:lactation; IEA:Ensembl.
DR GO; GO:0050821; P:protein stabilization; IEA:Ensembl.
DR InterPro; IPR000117; Casein_kappa.
DR PANTHER; PTHR11470; PTHR11470; 1.
DR Pfam; PF00997; Casein_kappa; 1.
DR PIRSF; PIRSF002374; Casein_kappa; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Milk protein; Phosphoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:4605338"
FT CHAIN 22..192
FT /note="Kappa-casein"
FT /id="PRO_0000004507"
FT REGION 165..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 126..127
FT /note="Cleavage; by chymosin/rennin"
FT MOD_RES 148
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT MOD_RES 172
FT /note="Phosphoserine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT MOD_RES 189
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02670"
FT CARBOHYD 152
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 155
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 156
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 159
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 172
FT /note="O-linked (GalNAc...) serine; alternate"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT CARBOHYD 188
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250|UniProtKB:P02668"
FT VARIANT 125
FT /note="S -> L"
FT /evidence="ECO:0000269|PubMed:15328285"
FT CONFLICT 23
FT /note="E -> Q (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 28
FT /note="Q -> E (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 192 AA; 21438 MW; 0FA87E633F939E58 CRC64;
MMKSFFLVVT ILALTLPFLG AQEQNQEQRI CCEKDERFFD DKIAKYIPIQ YVLSRYPSYG
LNYYQQRPVA LINNQFLPYP YYAKPVAVRS PAQTLQWQVL PNAVPAKSCQ DQPTAMARHP
HPHLSFMAIP PKKDQDKTEI PAINTIASAE PTVHSTPTTE AVVNAVDNPE ASSESIASAP
ETNTAQVTST EV
