ID   Y627_BARHE              Reviewed;         358 AA.
AC   Q8VQ25; Q6G5C4;
DT   15-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   31-AUG-2004, sequence version 2.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Putative zinc metalloprotease BH06270;
DE            EC=3.4.24.-;
GN   OrderedLocusNames=BH06270;
OS   Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)
OS   (Rochalimaea henselae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=283166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Zimmermann R., Augustin K., Schaal K., Sander A.;
RT   "Cloning, nucleotide sequencing, and expression of a hemin-binding protein
RT   of Bartonella henselae.";
RL   Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT   of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M50B family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAF27431.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF461795; AAL66373.1; -; Genomic_DNA.
DR   EMBL; BX897699; CAF27431.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011180551.1; NZ_LRIJ02000001.1.
DR   AlphaFoldDB; Q8VQ25; -.
DR   SMR; Q8VQ25; -.
DR   STRING; 283166.BH06270; -.
DR   PaxDb; Q8VQ25; -.
DR   PRIDE; Q8VQ25; -.
DR   EnsemblBacteria; CAF27431; CAF27431; BH06270.
DR   GeneID; 64156906; -.
DR   KEGG; bhe:BH06270; -.
DR   eggNOG; COG0750; Bacteria.
DR   Proteomes; UP000000421; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR004387; Pept_M50_Zn.
DR   InterPro; IPR008915; Peptidase_M50.
DR   PANTHER; PTHR42837; PTHR42837; 1.
DR   Pfam; PF13180; PDZ_2; 1.
DR   Pfam; PF02163; Peptidase_M50; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
DR   TIGRFAMs; TIGR00054; TIGR00054; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrolase; Membrane; Metal-binding;
KW   Metalloprotease; Protease; Transmembrane; Transmembrane helix; Zinc.
FT   CHAIN           1..358
FT                   /note="Putative zinc metalloprotease BH06270"
FT                   /id="PRO_0000088430"
FT   TRANSMEM        89..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          102..177
FT                   /note="PDZ"
FT   ACT_SITE        8
FT                   /evidence="ECO:0000255"
FT   BINDING         7
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
FT   BINDING         11
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        321
FT                   /note="T -> S (in Ref. 1; AAL66373)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   358 AA;  39715 MW;  AD99846E6FF1F704 CRC64;
     MIIIFVHEAG HYLIGRWCGI KASVFSLGFG PQIVGYTDKR GTQWRLALIP LGGYVKFIGD
     EEGLHGTSSQ SLPIVDGSFG SAHAWKKAAT VFAGPLFNVL FTVVILTFFF FTYGRVAIEP
     VVGSFVKDSP AVQAGLQLGD RFIEMDGQQV ESFEDLMNYV TFHGGDPIEF KMERSGQVFT
     TVITPKVVER DDGFGNRVRS GLMGVGVPVD PDNPARLDPA YVKHIRYSFG RALREASKRA
     TFIVTQTVFF MGRLLGGKED HCRLSGPSKT VKIAWQVSET GFLSLLNFTA FLSIGVGLIN
     LFPIPPLDGG YLLFHVVEII TGRPISAKIR EIIFRLGLCF VLLFMFFALF NDYFCWFS