ID   Y634_METJA              Reviewed;         620 AA.
AC   Q58051;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Uncharacterized KamA family protein MJ0634;
GN   OrderedLocusNames=MJ0634;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. KamA family.
CC       {ECO:0000305}.
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DR   EMBL; L77117; AAB98629.1; -; Genomic_DNA.
DR   PIR; B64379; B64379.
DR   RefSeq; WP_010870139.1; NC_000909.1.
DR   AlphaFoldDB; Q58051; -.
DR   SMR; Q58051; -.
DR   STRING; 243232.MJ_0634; -.
DR   EnsemblBacteria; AAB98629; AAB98629; MJ_0634.
DR   GeneID; 1451500; -.
DR   KEGG; mja:MJ_0634; -.
DR   eggNOG; arCOG03246; Archaea.
DR   HOGENOM; CLU_440512_0_0_2; -.
DR   InParanoid; Q58051; -.
DR   OMA; CQRMYDF; -.
DR   OrthoDB; 10285at2157; -.
DR   PhylomeDB; Q58051; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR003739; Lys_aminomutase/Glu_NH3_mut.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR30538; PTHR30538; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01070; PLP-dependent; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   TIGRFAMs; TIGR00238; TIGR00238; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Pyridoxal phosphate;
KW   Reference proteome; S-adenosyl-L-methionine.
FT   CHAIN           1..620
FT                   /note="Uncharacterized KamA family protein MJ0634"
FT                   /id="PRO_0000172295"
FT   DOMAIN          324..586
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         338
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         342
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         345
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         552
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   620 AA;  73372 MW;  4A5E12147E5F159C CRC64;
     MTIKSMTEYE TISYKTFLDI FSPLPEIGEI LEESESVEEA REKLFEFCKE LEWEIRMGRI
     KFDNEVDRWL ALKAIEVFLN IISKDNERLA GFSTLEYLWK AYKGDEEALK EIREGFIEEF
     RHLFLAMSGK ADYSLGFLGK RLLEEGVKFI DFSKIKGREA GIARSNFLDK VYEIMREYIS
     RYPSGLDKRI ILKRKKNREI LEEFFGITDE EWFNYKWQFK NVLRGLKGVK ILRELREVTN
     FKISDEDLEI IEKAVKNGIP FGLTPYYLHL FDFENPYVED LAVRRQVIPP EWYVEKMIEH
     KEDRNIAFDF MGEHDTSPID LVTRRYVTIA IIKPYESCPQ ICVYCQRNWM VQDFDAKAFP
     GWEKVEKALD WFAEHDSMIE ILITGGDPFS LSDKAIEKML NRIAEMNHVV GVRFGTRTIV
     TAPMRITDEL AELLGSFEKS LMISTHVESC YEITPEVAEA VKKLRTNNIY IYNQHVFHRY
     VSRRFENVAL RIALKKVGII PYYTFYPKGK MEHKDYLVPI ARLAQEVKEE ARLLPGSFRT
     DEPIFNVPRM GKNHLRAWQD RELIAIKPNG SRVYLMHPWE KGIYPTKLYT YEDVPIKEYL
     DSLKEIGENI EEYKTIWYYY