ID   Y6518_MYCS2             Reviewed;         129 AA.
AC   A0R6E3; I7FN93;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Uncharacterized protein MSMEG_6518/MSMEI_6344;
GN   OrderedLocusNames=MSMEG_6518, MSMEI_6344;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   PUPYLATION AT LYS-121, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20094657; DOI=10.1039/b916104j;
RA   Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA   Barry C.E. III, Bark S., Dorrestein P.C.;
RT   "Expansion of the mycobacterial 'PUPylome'.";
RL   Mol. Biosyst. 6:376-385(2010).
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP42770.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK72372.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42770.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003897932.1; NZ_SIJM01000033.1.
DR   RefSeq; YP_890731.1; NC_008596.1.
DR   AlphaFoldDB; A0R6E3; -.
DR   SMR; A0R6E3; -.
DR   STRING; 246196.MSMEI_6344; -.
DR   EnsemblBacteria; ABK72372; ABK72372; MSMEG_6518.
DR   EnsemblBacteria; AFP42770; AFP42770; MSMEI_6344.
DR   GeneID; 66737788; -.
DR   KEGG; msg:MSMEI_6344; -.
DR   KEGG; msm:MSMEG_6518; -.
DR   PATRIC; fig|246196.19.peg.6342; -.
DR   eggNOG; COG1716; Bacteria.
DR   OMA; QMFTASI; -.
DR   OrthoDB; 1689538at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Reference proteome; Ubl conjugation.
FT   CHAIN           1..129
FT                   /note="Uncharacterized protein MSMEG_6518/MSMEI_6344"
FT                   /id="PRO_0000396090"
FT   CROSSLNK        121
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20094657"
SQ   SEQUENCE   129 AA;  13362 MW;  3A619E1B32A1CEE2 CRC64;
     MYTAAIDALP PVGDAEFPER AAVVLSGLRK LQGSLAEAAS RSRATPSVIV ALSGVRTRYD
     ELMTTAAEGP GATLGQRLYV ARLRAKLTTA EAANGIGVRK DLIEAVEAEE PATEAETAQI
     KDLIAALGG