Y6545_BACAN
ID Y6545_BACAN Reviewed; 531 AA.
AC Q9RMZ0; Q8KYE8;
DT 02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Uncharacterized cell wall amidase pXO2-42/BXB0045/GBAA_pXO2_0045;
DE EC=3.5.1.-;
DE Flags: Precursor;
GN OrderedLocusNames=pXO2-42, BXB0045, GBAA_pXO2_0045;
OS Bacillus anthracis.
OG Plasmid pXO2.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Pasteur;
RX PubMed=10475962; DOI=10.1046/j.1365-2672.1999.00883.x;
RA Okinaka R.T., Cloud K., Hampton O., Hoffmaster A., Hill K.K., Keim P.,
RA Koehler T., Lamke G., Kumano S., Manter D., Martinez Y., Ricke D.,
RA Svensson R., Jackson P.J.;
RT "Sequence, assembly and analysis of pXO1 and pXO2.";
RL J. Appl. Microbiol. 87:261-262(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Ames / isolate Florida / A2012;
RX PubMed=12004073; DOI=10.1126/science.1071837;
RA Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA Fraser C.M.;
RT "Comparative genome sequencing for discovery of novel polymorphisms in
RT Bacillus anthracis.";
RL Science 296:2028-2033(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the N-acetylmuramoyl-
CC L-alanine amidase 3 family. {ECO:0000305}.
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DR EMBL; AF188935; AAF13647.1; -; Genomic_DNA.
DR EMBL; AE011191; AAM26204.1; -; Genomic_DNA.
DR EMBL; AE017335; AAT28975.2; -; Genomic_DNA.
DR RefSeq; NP_053197.1; NC_002146.1.
DR RefSeq; WP_001092801.1; NZ_VTZI01000012.1.
DR RefSeq; WP_010891437.1; NZ_CP009329.1.
DR AlphaFoldDB; Q9RMZ0; -.
DR SMR; Q9RMZ0; -.
DR EnsemblBacteria; AAT28975; AAT28975; GBAA_pXO2_0045.
DR GeneID; 45025353; -.
DR KEGG; bar:GBAA_pXO2_0045; -.
DR HOGENOM; CLU_029344_0_0_9; -.
DR OMA; NGFNEQV; -.
DR Proteomes; UP000000594; Plasmid pXO2.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:InterPro.
DR GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR CDD; cd02696; MurNAc-LAA; 1.
DR InterPro; IPR002508; MurNAc-LAA_cat.
DR InterPro; IPR001119; SLH_dom.
DR Pfam; PF01520; Amidase_3; 1.
DR Pfam; PF00395; SLH; 3.
DR SMART; SM00646; Ami_3; 1.
DR PROSITE; PS51272; SLH; 3.
PE 3: Inferred from homology;
KW Cell wall; Hydrolase; Plasmid; Reference proteome; Repeat; S-layer;
KW Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..531
FT /note="Uncharacterized cell wall amidase pXO2-
FT 42/BXB0045/GBAA_pXO2_0045"
FT /id="PRO_0000006467"
FT DOMAIN 29..85
FT /note="SLH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 86..149
FT /note="SLH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 150..210
FT /note="SLH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00777"
FT DOMAIN 335..517
FT /note="MurNAc-LAA"
FT /evidence="ECO:0000255"
FT VARIANT 27
FT /note="L -> S (in strain: Pasteur)"
FT VARIANT 311
FT /note="G -> S (in strain: Pasteur)"
SQ SEQUENCE 531 AA; 58860 MW; 46CBBFDD398DD7AC CRC64;
MNTKGIIAKL TAGALIANLL ICPANTLAEK KTFTDVPNWA QQSVNYLMKK ALDGKPDGTF
SPSEKIDRGS AAKLMAMVLG LQINKQAKPS FQDAKNHWAS PYIAAVEKAG VIYGDGSGNF
NPSKDIDRAS MASMLVEAYK LNNRIIGDLP TQFEDLKGHW GAKLANALVA LGISKGTGDG
WKPNGIVTRA EAVQFIAQTD MKKADTSKRM YMNRHFITYH QPSLSSGVTS NQHAPQIIVV
KEQRADGWIK IVTNIGDKWT PLYEKRETIH STFTTYPEAS HSSKVLGTHS PQTVTVIEEK
GSWIRIRTNA GFQWLDKNQL TLPKKQNNFL EGKTIIIDPG HGGIDGGHKG IYMNESPVVY
DTAVRVQKLF AQKTPFTALL TRDAYSRPGK NATDSLGKRV EFAKKNKGDI FVSIHANGFN
GNAHGTETFY YKAPTQKSNP YVNDSRILAE KIQKRLITAL QTRDRGVKIG NLYVLRENTM
PSVLTELGFV DNKADGKKLD SPEWRQRAAE AIYAGILDYY EWKGHNMSAY Y
