ID   Y6630_MYCS2             Reviewed;         116 AA.
AC   A0R6Q0; I7GBA9;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Uncharacterized protein MSMEG_6630;
GN   OrderedLocusNames=MSMEG_6630, MSMEI_6451;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   PUPYLATION AT LYS-76, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20094657; DOI=10.1039/b916104j;
RA   Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA   Barry C.E. III, Bark S., Dorrestein P.C.;
RT   "Expansion of the mycobacterial 'PUPylome'.";
RL   Mol. Biosyst. 6:376-385(2010).
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DR   EMBL; CP000480; ABK74785.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP42877.1; -; Genomic_DNA.
DR   RefSeq; WP_003898051.1; NZ_SIJM01000031.1.
DR   RefSeq; YP_890838.1; NC_008596.1.
DR   AlphaFoldDB; A0R6Q0; -.
DR   SMR; A0R6Q0; -.
DR   STRING; 246196.MSMEI_6451; -.
DR   EnsemblBacteria; ABK74785; ABK74785; MSMEG_6630.
DR   EnsemblBacteria; AFP42877; AFP42877; MSMEI_6451.
DR   GeneID; 66737894; -.
DR   KEGG; msg:MSMEI_6451; -.
DR   KEGG; msm:MSMEG_6630; -.
DR   PATRIC; fig|246196.19.peg.6454; -.
DR   eggNOG; COG0346; Bacteria.
DR   OMA; PYDDAHE; -.
DR   OrthoDB; 1864858at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   Gene3D; 3.10.180.10; -; 1.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR037523; VOC.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   PROSITE; PS51819; VOC; 1.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Reference proteome; Ubl conjugation.
FT   CHAIN           1..116
FT                   /note="Uncharacterized protein MSMEG_6630"
FT                   /id="PRO_0000396091"
FT   DOMAIN          5..113
FT                   /note="VOC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   CROSSLNK        76
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20094657"
SQ   SEQUENCE   116 AA;  12403 MW;  4E5D440CA555CBE6 CRC64;
     MSDHEVKMVV LSTENLDESI KFYETLGFSL KFRDGAHFAA LDGGAVTLAL ATPVDHPIPG
     KVVVGIKTAD VDAAAKEIEA TGGAIIKGPY DDAHERRAVV YDNTGNGLVF YSPLKR