CASP2_ARATH
ID CASP2_ARATH Reviewed; 204 AA.
AC Q9CAX3;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Casparian strip membrane protein 2;
DE Short=AtCASP2;
GN Name=CASP2; OrderedLocusNames=At3g11550; ORFNames=F24K9.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DIMERIZATION, AND INTERACTION WITH CASP1;
RP CASP3 AND CASP4.
RC STRAIN=cv. Columbia;
RX PubMed=21593871; DOI=10.1038/nature10070;
RA Roppolo D., De Rybel B., Denervaud Tendon V., Pfister A., Alassimone J.,
RA Vermeer J.E.M., Yamazaki M., Stierhof Y.-D., Beeckman T., Geldner N.;
RT "A novel protein family directs Casparian strip formation in the
RT endodermis.";
RL Nature 473:380-383(2011).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24920445; DOI=10.1104/pp.114.239137;
RA Roppolo D., Boeckmann B., Pfister A., Boutet E., Rubio M.C.,
RA Denervaud-Tendon V., Vermeer J.E., Gheyselinck J., Xenarios I., Geldner N.;
RT "Functional and evolutionary analysis of the CASPARIAN STRIP MEMBRANE
RT DOMAIN PROTEIN family.";
RL Plant Physiol. 165:1709-1722(2014).
CC -!- FUNCTION: Regulates membrane-cell wall junctions and localized cell
CC wall deposition. Required for establishment of the Casparian strip
CC membrane domain (CSD) and the subsequent formation of Casparian strips,
CC a cell wall modification of the root endodermis that determines an
CC apoplastic barrier between the intraorganismal apoplasm and the
CC extraorganismal apoplasm and prevents lateral diffusion.
CC {ECO:0000269|PubMed:21593871}.
CC -!- SUBUNIT: Homodimer and heterodimers with other CASP proteins. Interacts
CC with CASP1, CASP3 and CASP4. {ECO:0000269|PubMed:21593871}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:21593871};
CC Multi-pass membrane protein {ECO:0000269|PubMed:21593871}. Note=Very
CC restricted localization following a belt shape within the plasma
CC membrane which coincides with the position of the Casparian strip
CC membrane domain.
CC -!- SIMILARITY: Belongs to the Casparian strip membrane proteins (CASP)
CC family. {ECO:0000305}.
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DR EMBL; AC008153; AAG51441.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75062.1; -; Genomic_DNA.
DR EMBL; BT010843; AAR24210.1; -; mRNA.
DR EMBL; BT012612; AAT06431.1; -; mRNA.
DR RefSeq; NP_187762.1; NM_111988.3.
DR AlphaFoldDB; Q9CAX3; -.
DR SMR; Q9CAX3; -.
DR DIP; DIP-59178N; -.
DR IntAct; Q9CAX3; 3.
DR STRING; 3702.AT3G11550.1; -.
DR PaxDb; Q9CAX3; -.
DR PRIDE; Q9CAX3; -.
DR ProteomicsDB; 223866; -.
DR EnsemblPlants; AT3G11550.1; AT3G11550.1; AT3G11550.
DR GeneID; 820328; -.
DR Gramene; AT3G11550.1; AT3G11550.1; AT3G11550.
DR KEGG; ath:AT3G11550; -.
DR Araport; AT3G11550; -.
DR TAIR; locus:2080742; AT3G11550.
DR eggNOG; ENOG502QZV7; Eukaryota.
DR HOGENOM; CLU_066104_3_1_1; -.
DR InParanoid; Q9CAX3; -.
DR OMA; MPRRTHH; -.
DR OrthoDB; 1230007at2759; -.
DR PhylomeDB; Q9CAX3; -.
DR PRO; PR:Q9CAX3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9CAX3; baseline and differential.
DR Genevisible; Q9CAX3; AT.
DR GO; GO:0048226; C:Casparian strip; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0042545; P:cell wall modification; IMP:UniProtKB.
DR GO; GO:0007043; P:cell-cell junction assembly; IDA:UniProtKB.
DR InterPro; IPR006459; CASP/CASPL.
DR InterPro; IPR006702; CASP_dom.
DR Pfam; PF04535; DUF588; 1.
DR TIGRFAMs; TIGR01569; A_tha_TIGR01569; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cell wall biogenesis/degradation; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..204
FT /note="Casparian strip membrane protein 2"
FT /id="PRO_0000308666"
FT TOPO_DOM 1..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..92
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 204 AA; 21482 MW; 7EA15EF1A95E289E CRC64;
MKNESTFIDV PAESSSAMKG KAPLIGVARD HTTSGSGGYN RGLAIFDFLL RLAAIVAALA
AAATMGTSDE TLPFFTQFLQ FEASYDDLPT FQFFVIAMAL VGGYLVLSLP ISVVTILRPL
ATAPRLLLLV LDTGVLALNT AAASSAAAIS YLAHSGNQNT NWLPICQQFG DFCQKSSGAV
VSAFVSVVFF TILVVISGVA LKRH
