ID   Y681_MYCTU              Reviewed;         218 AA.
AC   O53789; I6XVZ1;
DT   12-AUG-2020, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 4.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=Uncharacterized HTH-type transcriptional regulator Rv0681 {ECO:0000305};
GN   OrderedLocusNames=Rv0681 {ECO:0000312|EMBL:CCP43424.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-15, AND SEQUENCE REVISION TO N-TERMINUS.
RC   STRAIN=H37Rv;
RX   PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA   Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA   Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT   "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT   annotated encoding genes.";
RL   Genomics 114:292-304(2021).
RN   [3]
RP   PHOSPHORYLATION AT THR-57, AND MUTAGENESIS OF THR-57.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=17286964; DOI=10.1016/j.bbrc.2007.01.122;
RA   Zheng X., Papavinasasundaram K.G., Av-Gay Y.;
RT   "Novel substrates of Mycobacterium tuberculosis PknH Ser/Thr kinase.";
RL   Biochem. Biophys. Res. Commun. 355:162-168(2007).
RN   [4] {ECO:0007744|PubMed:21969609}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- PTM: Phosphorylated on Thr-57 by PknH. {ECO:0000269|PubMed:17286964}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP43424.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR   EMBL; AL123456; CCP43424.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_215195.1; NC_000962.3.
DR   RefSeq; WP_003403451.1; NZ_NVQJ01000007.1.
DR   AlphaFoldDB; O53789; -.
DR   SMR; O53789; -.
DR   STRING; 83332.Rv0681; -.
DR   PaxDb; O53789; -.
DR   DNASU; 888239; -.
DR   GeneID; 45424643; -.
DR   GeneID; 888239; -.
DR   KEGG; mtu:Rv0681; -.
DR   PATRIC; fig|83332.111.peg.756; -.
DR   TubercuList; Rv0681; -.
DR   eggNOG; COG1309; Bacteria.
DR   InParanoid; O53789; -.
DR   OMA; IHETNQI; -.
DR   PhylomeDB; O53789; -.
DR   SABIO-RK; O53789; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProt.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   InterPro; IPR025996; TetR_C_WHG.
DR   Pfam; PF13305; TetR_C_33; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:34915127"
FT   CHAIN           2..218
FT                   /note="Uncharacterized HTH-type transcriptional regulator
FT                   Rv0681"
FT                   /id="PRO_0000450669"
FT   DOMAIN          28..88
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        51..70
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         57
FT                   /note="Phosphothreonine; by PknH"
FT                   /evidence="ECO:0000269|PubMed:17286964"
FT   MUTAGEN         57
FT                   /note="T->A: Almost completely abolishes phosphorylation by
FT                   PknH."
FT                   /evidence="ECO:0000269|PubMed:17286964"
SQ   SEQUENCE   218 AA;  23348 MW;  30DD65AE3A8FB829 CRC64;
     MAAQPQAPSA GGRPRAGKAV KSVARPAKLS RESIVEGALT FLDREGWDSL TINALATQLG
     TKGPSLYNHV DSLEDLRRAV RIRVIDDIIT MLNRVGAGRA RDDAVLVMAG AYRSYAHHHP
     GRYSAFTRMP LGGDDPEYTA ATRGAAAPVI AVLSSYGLDG EQAFYAALEF WSALHGFVLL
     EMTGVMDDID TDAVFTDMVL RLAAGMERRT THGGTAST