Y723_CHLP8
ID Y723_CHLP8 Reviewed; 327 AA.
AC O50310; B3QMI9;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Uncharacterized RNA pseudouridine synthase Cpar_0723;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase;
DE AltName: Full=RNA-uridine isomerase;
GN OrderedLocusNames=Cpar_0723;
OS Chlorobaculum parvum (strain DSM 263 / NCIMB 8327) (Chlorobium vibrioforme
OS subsp. thiosulfatophilum).
OC Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobaculum.
OX NCBI_TaxID=517417;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Petersen B.L., Moeller M.G., Stummann B.M., Henningsen K.W.;
RT "Clustering of genes with function in the biosynthesis of
RT bacteriochlorophyll and heme in the green sulfur bacterium Chlorobium
RT vibrioforme.";
RL Hereditas 125:93-96(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 263 / NCIMB 8327;
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Zhao F., Li T., Liu Z., Overmann J.,
RA Bryant D.A., Richardson P.;
RT "Complete sequence of Chlorobaculum parvum NCIB 8327.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACF11142.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z83933; CAB06297.1; -; Genomic_DNA.
DR EMBL; CP001099; ACF11142.1; ALT_INIT; Genomic_DNA.
DR PIR; T17190; T17190.
DR AlphaFoldDB; O50310; -.
DR SMR; O50310; -.
DR STRING; 517417.Cpar_0723; -.
DR EnsemblBacteria; ACF11142; ACF11142; Cpar_0723.
DR KEGG; cpc:Cpar_0723; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_4_3_10; -.
DR OMA; AHPSPGW; -.
DR Proteomes; UP000008811; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; RNA-binding.
FT CHAIN 1..327
FT /note="Uncharacterized RNA pseudouridine synthase
FT Cpar_0723"
FT /id="PRO_0000162732"
FT DOMAIN 12..84
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 137
FT /evidence="ECO:0000250"
SQ SEQUENCE 327 AA; 36736 MW; 549696105086A76E CRC64;
MTLDVSKVQT PMRIDRYLTQ QVENMTRNKV QAAIEEGRVL VNGKPVKSNY RVKSCDHIHL
TFLRPPAPEL APEDIPITIL YEDDDLMVID KEAGMVVHPA FGNWTGTLAN AILHHLGKDA
DELDKTEMRP GIVHRLDKDT SGLIIIAKNP TALHKLARQF ANRQVEKVYK AIVWGVPKAE
SGTIKTNIGR SHKNRKVMAN FPYEGAEGKH AITDYQVVED LGYFSLMDVT LHTGRTHQIR
VHLQHLGHSI LGDETYGGAT PRTLPFSKSE PFTRNLLELM SRQALHAQTL RFRQPTTGEP
LSFSAPLPED MELVLEKIRT VMTASQS