ID   Y728_ENTFA              Reviewed;         457 AA.
AC   Q837V0;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Uncharacterized RNA methyltransferase EF_0728;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=EF_0728;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE016830; AAO80548.1; -; Genomic_DNA.
DR   RefSeq; NP_814478.1; NC_004668.1.
DR   RefSeq; WP_002361260.1; NZ_KE136527.1.
DR   AlphaFoldDB; Q837V0; -.
DR   SMR; Q837V0; -.
DR   STRING; 226185.EF_0728; -.
DR   EnsemblBacteria; AAO80548; AAO80548; EF_0728.
DR   KEGG; efa:EF0728; -.
DR   PATRIC; fig|226185.45.peg.2669; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..457
FT                   /note="Uncharacterized RNA methyltransferase EF_0728"
FT                   /id="PRO_0000161978"
FT   DOMAIN          5..63
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        412
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         287
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         316
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         337
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         385
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   457 AA;  51250 MW;  136D1BB1937EE76E CRC64;
     MKNYPVKKND VIEVEIIDLT HEGLGVAKVD HYPLFIENAL PGEKLEIKVL KTGKSFGYGK
     VLTVLKSSEQ RVPVKDENFT KVGISPLQHL AYGAQLSFKT QQVENVMQRV AKLQEVPVLP
     TIGMNDPWHY RNKAQIPVRK IDNQLQTGFF RKNSHDLIPM EHFYIQDPEI DAAIVKIRDI
     MRKYSVKPYN ESDNTGNLRH IVVRRGYHTG EMMVVLITRT PKLFPISKIV PDILEAIPEV
     VSIVQNVNPK RTNVIFGDET ILLHGSEKIT DTIFDLKFEI SARSFYQVNP QQTEVMYQKV
     KEYAALTGNE IVVDAYCGIG TIGLTLAQDA KQVYGIEVIE EAVKDAENNA KLNNIENATF
     TAGLAEELLP KLVENGLQPD VVVVDPPRKG LDGQLVNTLI ETQPERIVYV SCNPATLARD
     IALLTEGGYE AKEIQPVDNF PQTTHIESVT LLTKAVD