ID   Y745_HELPJ              Reviewed;         327 AA.
AC   Q9ZL98;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase jhp_0682;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=jhp_0682;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE001439; AAD06270.1; -; Genomic_DNA.
DR   PIR; B71900; B71900.
DR   RefSeq; WP_001174338.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZL98; -.
DR   SMR; Q9ZL98; -.
DR   STRING; 85963.jhp_0682; -.
DR   EnsemblBacteria; AAD06270; AAD06270; jhp_0682.
DR   KEGG; hpj:jhp_0682; -.
DR   PATRIC; fig|85963.30.peg.297; -.
DR   eggNOG; COG0564; Bacteria.
DR   OMA; AHPSPGW; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; RNA-binding.
FT   CHAIN           1..327
FT                   /note="Uncharacterized RNA pseudouridine synthase jhp_0682"
FT                   /id="PRO_0000162736"
FT   DOMAIN          12..79
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        136
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   327 AA;  37722 MW;  7EDC7F6840D818BD CRC64;
     MQKVFIAPTH YKRIDEFLAK ELQISKNQVL NLIKEGLVFC QKKEVKKGGL ALKEGDAITL
     LTPKIVPKPL KKELDLEIEV IFEDEDLLVL NKPPNLVVHK APSVKEPTLV DWLKSQNYEL
     SNLGLKERYG IVHRLDKDTS GGIVIAKNNF THVCLSEQLK TKMMGRYYIA LLSTPLKEEK
     MSVECYLTRN PNNRLKMIAL KAAKKEKSRY SKSEFTSLLT SQNGMDLIGA KLFTGRTHQI
     RAHLEYLNRH IIGDNLYGLN GVLSKEEIRI MLHAYLIEFK HPRSEQKLRF KVPLLKDMLE
     YLKKVFDKEN LDEVLDEEKI LHAFIAK