ID   Y745_HELPY              Reviewed;         327 AA.
AC   O25441;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Uncharacterized RNA pseudouridine synthase HP_0745;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase;
DE   AltName: Full=RNA-uridine isomerase;
GN   OrderedLocusNames=HP_0745;
OS   Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700392 / 26695;
RX   PubMed=9252185; DOI=10.1038/41483;
RA   Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA   Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA   Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA   Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA   McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA   Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA   Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA   Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT   "The complete genome sequence of the gastric pathogen Helicobacter
RT   pylori.";
RL   Nature 388:539-547(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE000511; AAD07795.1; -; Genomic_DNA.
DR   PIR; A64613; A64613.
DR   RefSeq; NP_207538.1; NC_000915.1.
DR   RefSeq; WP_001174316.1; NC_018939.1.
DR   AlphaFoldDB; O25441; -.
DR   SMR; O25441; -.
DR   DIP; DIP-3289N; -.
DR   IntAct; O25441; 2.
DR   MINT; O25441; -.
DR   STRING; 85962.C694_03835; -.
DR   PaxDb; O25441; -.
DR   EnsemblBacteria; AAD07795; AAD07795; HP_0745.
DR   KEGG; hpy:HP_0745; -.
DR   PATRIC; fig|85962.47.peg.795; -.
DR   eggNOG; COG0564; Bacteria.
DR   OMA; AHPSPGW; -.
DR   PhylomeDB; O25441; -.
DR   Proteomes; UP000000429; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..327
FT                   /note="Uncharacterized RNA pseudouridine synthase HP_0745"
FT                   /id="PRO_0000162735"
FT   DOMAIN          12..79
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        136
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   327 AA;  37922 MW;  40E7F371C9E46117 CRC64;
     MQKVFIALTD HKRLDEFLAK ELQISKNQVL NLIKEGLVFC QKKEVKKGGL ALKEGDEITL
     LTPKITPKPL KKELDLEIEV IFEDEDLLVL NKPPNLVVHK ALSVKEPTLV DWLEFKNYEL
     SNLGLKERYG IVHRLDKDTS GGIVIAKNNF THVHLSEQLK TKMMGRYYIA LLSTPLKEEK
     MSVECYLTRN PNNRLKMIAL KAVKKEKSRY SKSEFISLLT SQNNLNLIGA KLFTGRTHQI
     RAHLEYLNRH IIGDNLYGLN GALPKEEIRI MLHAYLIEFK HPRSEQKLRF KVPLLKDMLE
     YLKKVFDKEN LDEVLDEEKI LHAFIAK