ID   Y77A_MYCSE              Reviewed;         377 AA.
AC   B3GNI9; A0A2U9PH69;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   22-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 19.
DE   RecName: Full=Uncharacterized protein D806_0077.1 {ECO:0000305};
GN   ORFNames=0070R {ECO:0000303|PubMed:18554329},
GN   D806_000760 {ECO:0000312|EMBL:AWT51070.1}, D806_0077.1 {ECO:0000305};
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be involved in the ESX-1 / type VII specialized secretion
CC       system (T7SS), which exports several proteins including EsxA and EsxB
CC       (Probable). Involved in DNA conjugation in the recipient strain
CC       (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain; strain still secretes EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: In the well characterized mc(2)155 strain this gene not
CC       present. {ECO:0000305}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AWT51070.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; EU711429; ACE06963.1; -; Genomic_DNA.
DR   EMBL; CP027541; AWT51070.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; B3GNI9; -.
DR   SMR; B3GNI9; -.
DR   EnsemblBacteria; AWT51070; AWT51070; D806_000760.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..377
FT                   /note="Uncharacterized protein D806_0077.1"
FT                   /id="PRO_0000438358"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          77..107
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   377 AA;  40379 MW;  F77AB8DB0E428E3B CRC64;
     MLAGLRRRGS MTTPPGPEIP PPHQGGFYSA GHHPQRPWPE TPPPKTRGGV KWMLGAVALL
     AVVGVTVAVT LAVTGKDKRD AIPPGSGVSG SPTASDIASA DDSGPVSVIT EDPTCAAQGP
     ILETFAAQQS QLWVERDPAL GRESWSPELR ADYEKVGKAM RTAADQVAQL AKITPHRAMR
     ELYEQFIAYA RAYADNIPNY TPPTDNLARV AVTAADAISY ICAAVSYGSA AARAPLVENR
     PAPTNVAPLG NPSEPERFLT APNPVCGEWS SVLNAFQTDT TEWLKTDPDI SSSQWSIEQK
     QINENVIPIM KRFANQLYLL GKDSGNPTFR DIADLSVQYR LAYVAAIPTY TPADKYLANA
     SIRLATMANV ACRAAAD