ID   Y7973_BRADU             Reviewed;         673 AA.
AC   Q89C25;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=UPF0313 protein blr7973 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=blr7973;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; BA000040; BAC53238.1; -; Genomic_DNA.
DR   RefSeq; NP_774613.1; NC_004463.1.
DR   RefSeq; WP_011090696.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89C25; -.
DR   STRING; 224911.27356258; -.
DR   PRIDE; Q89C25; -.
DR   EnsemblBacteria; BAC53238; BAC53238; BAC53238.
DR   GeneID; 64027720; -.
DR   KEGG; bja:blr7973; -.
DR   PATRIC; fig|224911.44.peg.8123; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_5; -.
DR   InParanoid; Q89C25; -.
DR   OMA; TCMYYTG; -.
DR   PhylomeDB; Q89C25; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..673
FT                   /note="UPF0313 protein blr7973"
FT                   /id="PRO_0000076380"
FT   DOMAIN          332..611
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          632..673
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         346
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         350
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         353
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   673 AA;  74982 MW;  2653DC396170EC16 CRC64;
     MDTQIIAAEK PLMAQARPRK PAPFLPMSRA EMDALGWDAC DIVLVTGDAY VDHPSFGMAI
     IGRLLEAQGF RVGIIAQPDW HSAEPLRALG KPKVFFGVTG GNMDSMVNRY TADRRLRHDD
     AYTAGGEGGK RPDRCTIVYA QRCREAFKDV PVVLGGIEAS LRRIAHYDYW SDKVRRSVLA
     DAKADLLLYG NAERAVVEVA NRLAAGEAPR ELDDIRGVAL FRRVPEDYSE LHADDLDSAD
     EGATRQKGAT VIRLPALEQV EQDKEAYARA SRVLHRESNP GNARPLVQRH GDRDLWLNPP
     PIPLTSDEMD AVYDLPYARA PHPSYGDAKI PAWDMIKFSV TIMRGCFGGC TFCSITEHEG
     RIIQNRSEGS ILREIEKIRD KTPGFTGVIS DIGGPTANMY RMACKDPKVE GACRRPSCVF
     PEICPNLNTS HDDLIRLYRK VRETKGIKKV MVASGVRYDL AVESPEYIKE LVTHHVGGYL
     KIAPEHTERG PLDKMMKPGI GAYNQFKRMF DAAAEQAGKK YYLIPYFIAA HPGTTDEDMM
     NLALWLKKNR YRADQVQTFL PSPMATATAM YHTGVNPLRG VRHGGSDKVE AIKGLRQRRL
     HKAFLRYHDP DNWPVLREAL KAMGRADLIG SRPDQLVPAH QPPGTGKAAG TRRPVRPGGK
     TQRFTTKGLR VMK