ID   CASP_ARATH              Reviewed;         689 AA.
AC   Q9LS42; Q93ZW7;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 2.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Protein CASP;
GN   Name=CASP; OrderedLocusNames=At3g18480; ORFNames=MYF24.37;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=12429822; DOI=10.1091/mbc.e02-06-0349;
RA   Gillingham A.K., Pfeifer A.C., Munro S.;
RT   "CASP, the alternatively spliced product of the gene encoding the CCAAT-
RT   displacement protein transcription factor, is a Golgi membrane protein
RT   related to giantin.";
RL   Mol. Biol. Cell 13:3761-3774(2002).
RN   [5]
RP   SUBCELLULAR LOCATION, TOPOLOGY, AND MUTAGENESIS OF TYR-648.
RX   PubMed=16028120; DOI=10.1007/s11103-005-4618-4;
RA   Renna L., Hanton S.L., Stefano G., Bortolotti L., Misra V., Brandizzi F.;
RT   "Identification and characterization of AtCASP, a plant transmembrane Golgi
RT   matrix protein.";
RL   Plant Mol. Biol. 58:109-122(2005).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Columbia;
RX   PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA   Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA   Rathjen J.P., Peck S.C.;
RT   "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT   thaliana.";
RL   J. Proteomics 72:439-451(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [8]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA   Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA   Giglione C.;
RT   "Comparative large-scale characterisation of plant vs. mammal proteins
RT   reveals similar and idiosyncratic N-alpha acetylation features.";
RL   Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC   -!- FUNCTION: May be involved in intra-Golgi transport. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:16028120}; Single-pass type IV membrane protein
CC       {ECO:0000269|PubMed:16028120}.
CC   -!- SIMILARITY: Belongs to the CASP family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB01114.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB026658; BAB01114.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE76102.1; -; Genomic_DNA.
DR   EMBL; AY056225; AAL07074.1; -; mRNA.
DR   EMBL; AY142680; AAN13218.1; -; mRNA.
DR   RefSeq; NP_566611.1; NM_112733.3.
DR   AlphaFoldDB; Q9LS42; -.
DR   SMR; Q9LS42; -.
DR   BioGRID; 6710; 14.
DR   IntAct; Q9LS42; 12.
DR   STRING; 3702.AT3G18480.1; -.
DR   iPTMnet; Q9LS42; -.
DR   PaxDb; Q9LS42; -.
DR   PRIDE; Q9LS42; -.
DR   ProteomicsDB; 223884; -.
DR   EnsemblPlants; AT3G18480.1; AT3G18480.1; AT3G18480.
DR   GeneID; 821377; -.
DR   Gramene; AT3G18480.1; AT3G18480.1; AT3G18480.
DR   KEGG; ath:AT3G18480; -.
DR   Araport; AT3G18480; -.
DR   TAIR; locus:2095012; AT3G18480.
DR   eggNOG; KOG0963; Eukaryota.
DR   HOGENOM; CLU_016758_0_0_1; -.
DR   InParanoid; Q9LS42; -.
DR   OMA; DNWEAKE; -.
DR   OrthoDB; 1081139at2759; -.
DR   PhylomeDB; Q9LS42; -.
DR   PRO; PR:Q9LS42; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LS42; baseline and differential.
DR   Genevisible; Q9LS42; AT.
DR   GO; GO:0005768; C:endosome; HDA:TAIR.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR   GO; GO:0030173; C:integral component of Golgi membrane; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IDA:TAIR.
DR   GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:InterPro.
DR   InterPro; IPR012955; CASP_C.
DR   Pfam; PF08172; CASP_C; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Coiled coil; Golgi apparatus; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..689
FT                   /note="Protein CASP"
FT                   /id="PRO_0000071794"
FT   TOPO_DOM        1..643
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        644..661
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        662..689
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          49..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          278..307
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          79..367
FT                   /evidence="ECO:0000255"
FT   COILED          410..467
FT                   /evidence="ECO:0000255"
FT   COILED          514..556
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        10..27
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..76
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        278..301
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0007744|PubMed:22223895"
FT   MUTAGEN         648
FT                   /note="Y->L: No effect on subcellular location when
FT                   expressed in tobacco cells; retained in the endoplasmic
FT                   reticulum when expressed in mammalian cells."
FT                   /evidence="ECO:0000269|PubMed:16028120"
SQ   SEQUENCE   689 AA;  79684 MW;  D05C69CFF3303DCE CRC64;
     MEVSQDGSER DKTPPPSSSS SSSSPIPVVT NFWKEFDLEK EKSLLDEQGL RIAENQENSQ
     KNRRKLAEST RDFKKASPEN KLSMFNSLLK GYQEEVDNIT KRAKFGENAF LNIYQKLYEA
     PDPFPALASI AEQDRKLSEV ESENRKMKVE LEEFRTEATH LKNQQATIRR LEERNRQLEQ
     QMEEKIKEVV EIKQRNLAEE NQKTMELLKD REQALQDQLR QAKDSVSTMQ KLHELAQNQL
     FELRAQSDEE TAGKQSEVSL LMDEVERAQT RLLTLEREKG HLRSQLQTAN EDTDNKKSDN
     IDSNSMLENS LTAKEKIISE LNMEIHNVET ALANERESHV AEIKKLNSLL NKKDTIIEEM
     KKELQERPSA KLVDDLRKKV KILQAVGYNS IEAEDWDAAT TGEEMSKMES LLLDKNRKME
     HEVTQLKVQL SEKASLLEKA EAKGEELTAK VNEQQRLIQK LEDDILKGYG SKERKGALFD
     EWEFSEAGVA EQSEPMDQKH VPSEQDQSSM LKVICSQRDR FRARLRETEE EIRRLKEKIG
     FLTDELEKTK ADNVKLYGKI RYVQDYNHDK VVSRGSKKYV EDLESGFSSD VESKYKKIYE
     DDINPFAAFS KKEREQRIKD LGIRDRITLS SGRFLLGNKY ARTFAFFYTI GLHVLVFTCL
     YRMSAYSYLS HGAEETLMTE ATTNLPHGL