ID   Y819_PYRHO              Reviewed;         459 AA.
AC   O58549;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Uncharacterized methyltransferase PH0819;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=PH0819;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BA000001; BAA29912.1; -; Genomic_DNA.
DR   PIR; F71131; F71131.
DR   AlphaFoldDB; O58549; -.
DR   SMR; O58549; -.
DR   STRING; 70601.3257229; -.
DR   EnsemblBacteria; BAA29912; BAA29912; BAA29912.
DR   KEGG; pho:PH0819; -.
DR   eggNOG; arCOG01356; Archaea.
DR   OMA; GNWPPAW; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.30.20; -; 1.
DR   InterPro; IPR006158; Cobalamin-bd.
DR   InterPro; IPR036724; Cobalamin-bd_sf.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR034466; Methyltransferase_Class_B.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   Pfam; PF02310; B12-binding; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00729; Elp3; 1.
DR   SUPFAM; SSF52242; SSF52242; 1.
DR   PROSITE; PS51332; B12_BINDING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Methyltransferase;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..459
FT                   /note="Uncharacterized methyltransferase PH0819"
FT                   /id="PRO_0000184778"
FT   DOMAIN          13..145
FT                   /note="B12-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00666"
FT   DOMAIN          188..402
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         202
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         206
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         209
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  51718 MW;  766BD6444E27C414 CRC64;
     MKVMKILLVL PPTESAIKRV VGTTGPPLGL AYLASMVREE HDVKIIDGLA EDLTFSDIAK
     IIKKFDPDIV GITATTSAMY DAYTVAKIAK NINENVFVVM GGPHVTFTPE LTMRECPCID
     AVVRGEGELT FKELVDALSK GRELKGILGL SYKENGKVRN EPPRPLIQNV DEIPIPSYDL
     LPMDKYKADG VPFGVVMTSR GCPFNCVFCS SSLQFGKRWR GHSVERVIEE LSILHYEYGI
     KEIEFLDDTF TLNKKRAIDI SLRIKQEGLD ISWTASSRVN TFNEKVAKAM KEGGCHTVYF
     GIESASPRIL EFIGKGITPQ QSIDAVKTAK KFGLHALGSF IIGFPDETRE EVEATIKFAK
     KLDIDYAQFT IATPYPGTRL WEYAIANNLL LTMNWRKYTT IDPVMKLKHF TSEQISKLLR
     KAYISFYLRP KVLIKDIFEH HGFIIKRAIR GLLRVYYQA