ID   Y832_METJA              Reviewed;        1750 AA.
AC   Q58242;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Uncharacterized protein MJ0832;
DE   Contains:
DE     RecName: Full=Mja rnr-1 intein;
DE   Contains:
DE     RecName: Full=Mja rnr-2 intein;
GN   OrderedLocusNames=MJ0832;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
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DR   EMBL; L77117; AAB98834.1; -; Genomic_DNA.
DR   PIR; H64403; H64403.
DR   AlphaFoldDB; Q58242; -.
DR   SMR; Q58242; -.
DR   STRING; 243232.MJ_0832; -.
DR   EnsemblBacteria; AAB98834; AAB98834; MJ_0832.
DR   KEGG; mja:MJ_0832; -.
DR   eggNOG; arCOG03145; Archaea.
DR   eggNOG; arCOG03154; Archaea.
DR   eggNOG; arCOG03714; Archaea.
DR   eggNOG; arCOG04889; Archaea.
DR   HOGENOM; CLU_002707_0_0_2; -.
DR   InParanoid; Q58242; -.
DR   OMA; CQFLGLF; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0031250; C:anaerobic ribonucleoside-triphosphate reductase complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0008998; F:ribonucleoside-triphosphate reductase activity; IBA:GO_Central.
DR   GO; GO:0009265; P:2'-deoxyribonucleotide biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   Gene3D; 3.10.28.10; -; 2.
DR   InterPro; IPR005144; ATP-cone_dom.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR004860; LAGLIDADG_2.
DR   InterPro; IPR012833; NrdD.
DR   Pfam; PF03477; ATP-cone; 2.
DR   Pfam; PF14528; LAGLIDADG_3; 2.
DR   Pfam; PF13597; NRDD; 3.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 2.
DR   SMART; SM00306; HintN; 2.
DR   SUPFAM; SSF51294; SSF51294; 2.
DR   SUPFAM; SSF55608; SSF55608; 3.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 2.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 2.
DR   PROSITE; PS51161; ATP_CONE; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 2.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 2.
DR   PROSITE; PS50817; INTEIN_N_TER; 2.
PE   4: Predicted;
KW   ATP-binding; Autocatalytic cleavage; Nucleotide-binding; Protein splicing;
KW   Reference proteome; Repeat.
FT   CHAIN           1..337
FT                   /note="Uncharacterized protein MJ0832, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013999"
FT   CHAIN           338..790
FT                   /note="Mja rnr-1 intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014000"
FT   CHAIN           791..1058
FT                   /note="Uncharacterized protein MJ0832, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014001"
FT   CHAIN           1059..1591
FT                   /note="Mja rnr-2 intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014002"
FT   CHAIN           1592..1750
FT                   /note="Uncharacterized protein MJ0832, 3rd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000014003"
FT   DOMAIN          14..101
FT                   /note="ATP-cone"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00492"
FT   DOMAIN          507..634
FT                   /note="DOD-type homing endonuclease 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
FT   DOMAIN          1286..1426
FT                   /note="DOD-type homing endonuclease 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ   SEQUENCE   1750 AA;  203281 MW;  6D6893B770A25359 CRC64;
     MISAKDFAEK VMEFYVIKRD KRKEKFNVNK LAKSLINSGV NYGDLDTIIS EVCAKVYNGI
     TTDELKDIVY NVLKKIDKDV AENYRNGIIL KVRTSEKEFE SFDKEKIAKA LIRETGADEE
     TARKIADEVE RELKKLKVKY LTAPMIREIV NYKLIEYGFE ELRHKHTRLG MPVYDITKLI
     KSGSRENANL MYNPESIHKW VADETMKQYA LLAIFPKHIA DAHIKGDIHL HDLEYAATRP
     VCLQHDLRPF FKYGLKVDGT GLHTSVSKPA KHPEVAIQHA AKVMMAAQTN MSGGQSIDEF
     NVWLAPYVRG LSYEKIKQLM QMFIYELNQM YVARGGQSLG RDELIFIKEG DKLKVCKIGE
     AIDEFMEKYK DKIIVDGDTE ILYLDGIAEV YTISVNVKTG KAEFKRVYAI SRHKPRGKVY
     KVIGKDGTSI IVTEDHSLFN YDENGNLVCV KPRQMKHIIR NFNNPYDVEY RIGDYIETNY
     QRTDSKYNSR QNDIPEKLKI TKELCQFLGL FVAEGSYITN GISITTKDDD IAKFIERFVK
     EQINENIAVK RYEDSVRFVN KGFYRFLKEH INGKAINKNS PEFILKGDKE MKLAFLGGLI
     SGDGYVSKDG RVQIYTTSEQ LLGQLHLLLS DLGMIYSITK IKEEGEKIEI KRNEIVRNYK
     LYVIEIAKNC TEDLKPYVIP KYKKERIKPA NYDQLPYDYR IIKEHLRKIT DKKPYNDYAW
     KSNNRKLKLN TLEKIEQLNP HLREEINKFK LNIPFEIKEI KEIDYNGYVY DLSVEDNENF
     ITATGILCHN TIFSSINLEL EIPEFLKDKP AVIAGTTRGT YGDYEEEAKL ILEALVDVMM
     EGDAMGKPFL FPNFIIKLRE NAFKDENKEL MYKIHQLSAK FGIPYFINML PDWQVTNTNA
     MGCRTRLSGN WTGDAEIDTL RTGNMQWYSL NLPRIAYEAN GDDTKLFEIL HERLEILKEA
     LLIKHEVTKE RLYVDNLMPF LTQEFDGESY YRYENTTKTF GFVGLNEMLK YHLGEELHES
     KDAVKFGEKV IEYIREYADK LKEETGLRWT VTQTPAESSL PYDEKILIFE NNEYKLVKIG
     EFVEKYLNRY KDRAITYGDN NIEVYIKDEN IYAPSFDKDG KIVLKPITHA IRHRGKEIYE
     IELESGKKVR VTGDHSVFTI NDNLDVVEVK ASDLKVGDFI ITPKIIPSIS KDKIYLSEIV
     KNKDKYYVKI KDHIKFIEEH EEILKESYKE YKTKWKDLKP VLKKKNAFRL DLIEDLVDKE
     KIEKISYGHA NYINNKIKLD EKFGYLIGAF LSEGHWNDKC VEISSTNKEF IENLVEIIEE
     ILGKDAYYIT VKGDKRRYKD LYVIGLNKTV AMIFESLGLN KLSSNKEIPS ILLSNETFLK
     GLIKGYIDGD GSIYVDESKR DYSIRLYTTS ETLRDTLCLA LKILGINYRL SIDKKSKVNE
     NWRDCYVIKI TGKENIEKLL DVEIKNNGGK DVIPKIAEKF KEIINQYSQR EWKERFGIDV
     NNLHIWEDLK KGYMSRYRAK KVLNIMKNVK EIEEKYGRLL DKIGQLIDND LLFERIKSIR
     VLDEIPEYVY DISVEGTENF IGGEGFICLH NTAGRFARLD YKYYKEETIS VVRGDLNDVD
     SLYYTNSSHV RVDAPITLGE KVRIEEKFHP LCNGGHIMHI WNIESAADPE VLMDITKKIT
     KTHIGFWTYT KNLSVCNRCG ISMGGLRDRC INCGSEDVAK FSRITGYLQN ISNWNRAKQK
     ELEDRKLPRI