ID   CASS_KALDA              Reviewed;         764 AA.
AC   E2IUB0;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Cycloartenol synthase;
DE            Short=KdCAS;
DE            EC=5.4.99.8;
OS   Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Saxifragales; Crassulaceae; Kalanchoe.
OX   NCBI_TaxID=23013;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=20610397; DOI=10.1074/jbc.m109.098871;
RA   Wang Z., Yeats T., Han H., Jetter R.;
RT   "Cloning and characterization of oxidosqualene cyclases from Kalanchoe
RT   daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding
RT   friedelin and other triterpenoids.";
RL   J. Biol. Chem. 285:29703-29712(2010).
CC   -!- FUNCTION: Oxidosqualene cyclase which catalyzes the conversion of
CC       oxidosqualene to cycloartenol. {ECO:0000269|PubMed:20610397}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC         Evidence={ECO:0000269|PubMed:20610397};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in all leaf tissues.
CC       {ECO:0000269|PubMed:20610397}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; HM623872; ADK35127.1; -; mRNA.
DR   AlphaFoldDB; E2IUB0; -.
DR   SMR; E2IUB0; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016871; F:cycloartenol synthase activity; IDA:UniProtKB.
DR   GO; GO:0006694; P:steroid biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Lipid biosynthesis; Lipid metabolism; Membrane; Repeat;
KW   Steroid biosynthesis; Transmembrane; Transmembrane helix.
FT   CHAIN           1..764
FT                   /note="Cycloartenol synthase"
FT                   /id="PRO_0000418479"
FT   TRANSMEM        609..629
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          513..558
FT                   /note="PFTB 2"
FT   REPEAT          590..630
FT                   /note="PFTB 3"
FT   REPEAT          639..680
FT                   /note="PFTB 4"
FT   REPEAT          701..742
FT                   /note="PFTB 5"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   764 AA;  86531 MW;  9A59D94C1072CF6A CRC64;
     MWKLKIADAG GSQWLRSVNN HIGRQIWDFD PALGSPEELA QIEDARDNFA RHRFDKKHSA
     DLLMRFQLTK ENPQSDLLPK VNIGKIEDIT EDAVTNTLRR AINFHSTTQA HDGHWPGDYG
     GPLFLMPGLV ITLSITGALN AVLSKEHKKE MCRYLYNHQN EDGGWGLHIE GPSTMFGSVL
     NYVTLRLLGE DVNGGDGEIE RARKWILDHG GATAITSWGK MWLSVLGVFE WCGNNPLPPE
     MWLFPYYLPV HPGRMWCHCR MVYLPMSYLY GKRFVGPITP TVLSLRKELF TVPYHEIDWN
     EARSLCAKED LYYPHPVVQD ILWATLHKVV EPVLLNWPGK KLREKALCSA IEHIHYEDEN
     TRYICIGPVN KVLNMLCCWV EDPNSEAFKL HIPRLYDYLW IAEDGMKMQG YNGSQLWDTA
     FSVQAIVATK LVEEFSSTIS KAHEFMKNSQ VLEDYPGDLS YWYRHISKGA WPFSTADHGW
     PISDCTAEGL KVVLKLSQFP AELVGAPLSA KLVYNAVNVI LSLQNIDGGF ATYELTRSYS
     WMELLNPAET FGDIVIDYPY VECTSAALQS LVLFKKLHPE HRKEEVELCI KKAAAFIEKI
     QESDGSWYGS WAVCFTYGTW FGVLGLVAAG RNYKNSPSIR KACDFLLSKQ LASGGWGESY
     LSCQNKVYTN IPGGRSHVVN TGWAMLALIG AGQAERDPVP LHRAAKFLIE SQLENGDFPQ
     QEIMGVFNKN CMISYAAYRN IFPIWALGEY RCKVLNASRG QMKT